环境中的抗生素Antibioticsin the .ppt

* * * * * * * * * * * * * * * * * * * * * * Our Procedure “Delvotest P-Mini” Sensitive to many antibiotics Initial kit comes with several boxes worth of tests along with a block heater for ~ $100. Cost of 25 ampules = ~ $30 - $35 Color Change takes ~ 2 – 2.5 hours Advantages over Premitest Initial tests indicate no additional materials needed Comes with “nutrient pellets” Procedure Step 1: Preparation Cut off the required number of ampoules with a pair of scissors. Be careful not to damage the foil of the remaining ampoules. Step 2: Open the seal Open ampoule(s) by punching a hole in the aluminum foil with the syringe. Mark the ampoules for sample identification. Step 3: Take a milk water sample Attach a new disposable pipette to the syringe. Depress the plunger completely, dip the tip in the milk sample and allow the plunger to return slowly under pressure of the spring. Procedure Step 4: Inoculation Empty the syringe into the correspondingly marked ampoule by slowly depressing the plunger of the syringe. Use a fresh disposable pipette for each sample. Step 5: Incubation Check the temperature of the incubator (64oC ±0.5oC). Put the ampoule(s) into the incubator. Record the time and set timer for 3 hours or use control time. Step 6: Results After 3 hours, remove the samples from the incubator. Read the colour of the lower 2/3 part of the solid agar in the ampoule(s) after the required incubation time. Data Analysis Collect digital image of the vials Digital Photo Image Scanner Create .pdf file Analyse image for color using “colors.exe” /pica.html Otaka I, Kumagai K, Inagaki Y, Shimoyama M, Saegusa K, Hara T (2002) Simple and inexpensive software designed for the evaluation of color American Journal of Ophthalmology 133 (1): 140 – 142. Vector Analysis to quantify response Digital Images Positive Control Negative Control “Real Images” Full Test (Amoxicillin in DI water) Images of groups of vials 0.5, 1, 1 and 4 μg/L 6, 8, 10 and 20 μg/L Positive contro