to bioinformatics（生物信息学国外教程2010版）课件.pptVIP

* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * Entrez Gene result for a histone Outline for today Definition of bioinformatics Overview of the NCBI website Accessing information: accession numbers and RefSeq Entrez Gene (and UniGene, HomoloGene) Protein Databases: UniProt, ExPASy Three genome browsers: NCBI, UCSC, Ensembl Access to biomedical literature PubMed at NCBI to find literature information PubMed is the NCBI gateway to MEDLINE. MEDLINE contains bibliographic citations and author abstracts from over 4,600 journals published in the United States and in 70 foreign countries. It has 20 million records dating back to 1950s. Page 38 MeSH is the acronym for Medical Subject Headings. MeSH is the list of the vocabulary terms used for subject analysis of biomedical literature at NLM. MeSH vocabulary is used for indexing journal articles for MEDLINE. The MeSH controlled vocabulary imposes uniformity and consistency to the indexing of biomedical literature. Page 38 PubMed result for HBB Use the pull-down menu to access related resources such as Medical Subject Headings (MeSH) A “how to” pull-down menu links to tutorials Use “Advanced search” to limit by author, year, language, etc. PubMed search strategies Try the tutorial Use boolean queries (capitalize AND, OR, NOT) lipocalin AND disease Try using limits (see Advanced search) There are links to find Entrez entries and external resources Obtain articles on-line via Welch Medical Library (and download pdf files): / Page 39 lipocalin AND disease (504 results) lipocalin OR disease (2,500,000 results) lipocalin NOT disease (2,370 results) 1 AND 2 1 OR 2 1 NOT 2 1 1 1 2 2 2 Page 40 WelchWeb is available at Welch Medical Library liasons to the basic sciences WelchWeb is available at Reminder: Please enroll! Google “moodle bioinformatics” to get here; click “Bioinformatics” to sign in; The enrollment key is… * * * * * * *