抗生素英文课件精品cultivation and transformation of .pptVIP

Tian He Media non-selective: YPD without antibiotics Selective: Synthetic complete dropout medium (SC) (auxotroph) YPD with antibiotics YPD: 1000 mL Yeast Extract 10 g Peptone (Tryptone) 20 g Glucose 20 g Agar (for plates) 20 g Distilled H2O 1000 mL Synthetic complete dropout medium (SC) 1000 mL Yeast nitrogen base without amino acids (YNB) 6.7 g Dropout mix (-His/-Trp/-Leu DO mix) 0.62 g Amino acids Glucose