土壤微生物与抗生素（英文PPT）Organisms in Soil and Antibiotics课件.ppt

Soil Microorganisms and Antibiotics December 6, 2004 Kenice Frank, Allison Johnson, Ruben Krantz, Hannah Wilbur Soil bacteria Habitats: Water films Need water for metabolic processes On surface of organic matter Need surface on which to grow In rhizosphere Competition Compete with surrounding bacteria and fungi Production of antibiotics by competing bacteria Antibiotics Can be used as: Fungicide, etc for farming Antibiotics for humans and animals From Strepomyces species alone: 500 antibacterial products identified Objectives Identify through laboratory testing members of the soil communities Isolate and cultivate antibacterial producing bacteria from soil Observe and understand members of different communities of soil bacteria Soil Experiment Methods and Materials Methods and Materials Methods and Materials We added sterilized H2O to each soil sample and streaked 500 ml of each soil type onto 4 starch-casein agar for each soil type. We incubated these plates at room temperature. Methods and Materials Week 2 We took 100 ml of forest sample and diluted it with 400 ml more of sterile H2O We isolated 4 visually different colonies from each soil type and streaked each colony type onto individual starch casein agar -We did a smear of the entire forest plate and the entire river plate because there were no identifiable, separate colonies Incubate at room temperature Methods and Materials Week 3 Got results from previous week’s plates examined for any Streptomyces by looking for any areas of inhibition (clear areas surrounding colonies) Using soil plate prepared for the general lab, we tried to isolate antibiotic producing organism again Methods and Materials We streaked the lab specimen and a sample from each soil plate from last week down the middle of a BAP (blood agar plate) and then used method for testing sensitivity to observe for any areas of inhibition or hemolysis We then streaked S.aureus and E.coli on starch casin agar plates perpendicularly t