代谢工程 英文原版PDF Chapter_4_-_Material_Balances_and_Data_Cons.pdfVIP

C H A P T E R 4 Material Balances and Data Consistency Quantitative analysis of metabolism requires experimental data for the determination of metabolic fluxes, flux distributions, and measures of flux control (see Chapter 11), among other parameters. As such, these calcula- tions exemplify methods and procedures for upgrading the information con- tent of primary fermentation data. Whereas our focus in this book is on metabolism and its control, the basic philosophy of information content upgrade is applicable throughout the life sciences, so long as quantitative measurements are available. Because these methods of information upgrade are data-driven, it is of the utmost importance to ensure the reliability of the data used. This can be done by applying the usual methods of random error minimization, i.e. use of repeat experiments, multiple sensors, careful calibrations, etc. An addi- tional consideration (which is the subject of this chapter) is the introduction of data redundancy for the validation of both the actual measurements, and the broader mechanistic framework within which such measurements are 115 116 Metabolic Engineering analyzed. For example, in the context of metabolic analysis, flux calculations are based on the measurement of the specific rates for substrate uptake and product formation, which represent the fluxes in and out of the cells. Before any such derivative calculations are carried out, it is important that the consistency of