甲醛胁迫对洋葱内表皮细胞凋的诱导生物毕业论文.docVIP

甲醛胁迫对洋葱内表皮细胞凋亡的诱导 目录 1.论文题目、姓名、学院........................................... 2 2. 中文摘要、英文摘要...........................................2-3 3. 引言..........................................................3 4.实验材料与方法步骤:.............................................3 4.1实验材料..................................................................................... 3 4.2方法步骤：.................................................3 4.2.1材料处理...............................................3 4.2.1.1预处理...........................................3 4.2.1.2取材.............................................4 4.2.1.3处理.............................................4 4.2碱性品红溶液染色及形态学观测................................4 4.3细胞凋亡计数统计............................................4 结果与分析......................................................4 5.1细胞凋亡的形态学变化.....................................4-5 5.2细胞凋亡率................................................5 讨论..........................................................5-6 参考文献......................................................6-7 致谢...........................................................7 甲醛胁迫对洋葱内表皮细胞调往的诱导 摘要： 细胞凋亡是当今环境毒理学领域的研究热点之一．为了探讨甲醛能否诱导细胞凋亡，以洋葱内表皮细胞为实验材料，通过不同浓度的甲醛处理一段时间后，应用形态学观察、显微镜观察实验检测了洋葱内表皮细胞凋亡情况．实验结果表明低浓度甲醛 (≤0．2mmol·L0．4mmol·L细胞萎缩成圆形， 且会造成细胞核DNA有规律的断裂，细胞凋亡率随甲醛浓度Abstract： Nowadays apoptosis has gained much interest in the environmentaltoxicology．In order to explore whether formaldehyde could induce apoptosis or not， Onion epidermal cells were treated with differentconcentrations of fomraldehyde for a period of time ．Morphologic observationMicroscopic observation of experimental were applied to detect the apoptosis of Onion epidermal cells in this experiment．Results showed that Onion epidermal cells’ prolfeation was significantly inhibited alter treated with FA atlow concentrations (≤ 0．2mmolL )ina period of time ，while morphological change of cells was negligible，and n