Periscope_ quantitative prediction镜定量预测.pdfVIP

/scientificreports OPEN Periscope: quantitative prediction of soluble protein expression in the periplasm of Escherichia coli Received: 02 November 2015 1,3 3 4 1,2 3,4,6 Catherine Ching Han Chang , Chen Li , Geoffrey I. Webb , BengTi Tey , Jiangning Song accepted: 28 January 2016 Ramakrishnan Nagasundara Ramanan1,2,5 Published: 02 March 2016 Periplasmic expression of soluble proteins in Escherichia coli not only offers a much-simplified downstream purification process, but also enhances the probability of obtaining correctly folded and biologically active proteins. Different combinations of signal peptides and target proteins lead to different soluble protein expression levels, ranging from negligible to several grams per litre. Accurate algorithms for rational selection of promising candidates can serve as a powerful tool to complement with current trial-and-error approaches. Accordingly, proteomics studies can be conducted with greater efficiency and cost-effectiveness. Here, we developed a predictor with a two-stage architecture, to predict the real-valued expression level of target protein in the periplasm. The output of the first-stage support vector machine (SVM) classifier determines which second-stage support vector regression (SVR)