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Porous Silicon-Based Biosensors_生物传感器.pdf

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/scientificreports OPEN Porous Silicon-Based Biosensors: Towards Real-Time Optical Detection of Target Bacteria in the Received: 20 June 2016 Food Industry Accepted: 04 November 2016 Published: 30 November 2016 1,* 2,* 1 3 Naama Massad-Ivanir , Giorgi Shtenberg , Nitzan Raz , Christel Gazenbeek , 4 3 1,5 Dries Budding , Martine P. Bos Ester Segal Rapid detection of target bacteria is crucial to provide a safe food supply and to prevent foodborne diseases. Herein, we present an optical biosensor for identification and quantification of Escherichia coli (E. coli, used as a model indicator bacteria species) in complex food industry process water. The biosensor is based on a nanostructured, oxidized porous silicon (PSi) thin film which is functionalized with specific antibodies against E. coli. The biosensors were exposed to water samples collected directly from process lines of fresh-cut produce and their reflectivity spectra were collected in real time. Process water were characterized by complex natural micro-flora (microbial load of 107 cell/mL), in addition to soil particles and plant cell debris. We show that process water spiked with culture-grow