毛细管电泳法测定麻黄及麻杏石甘汤中主成分含量.docVIP

毛细管电泳法测定麻黄及麻杏石甘汤中主成分的含量 摘要:建立了毛细管区带电泳法测定麻黄及麻杏石甘汤中麻黄碱和伪麻黄碱含量的方法。电泳条件：电泳缓冲液为50 mmol/L硼砂-20 mmol/L苏氨酸缓冲溶液(1 mol/LNaOH溶液调pH至9.27)；紫外检测波长210 nm，分离电压15 kV；以苯巴比妥为内标物，采用压力进样（10 cm×20 s），柱温：室温。麻黄碱与伪麻黄碱的线性范围分别为21.3～213 μg/mL和8.4～84 μg/mL，相关系数分别为0.9996和0.9995，检测限分别为1.45 μg/mL和1.48 μg/mL，定量限分别为4.81 μg/mL和4.93 μg/mL。将该法用于麻黄中麻黄碱与伪麻黄碱的含量测定，平均回收率分别为97.5%和98.6%。本法操作简便、快速、经济、灵敏度高，麻黄及麻杏石甘汤中麻黄碱及伪麻黄碱可获得满意的分离，可作为麻黄和麻杏石甘汤中麻黄碱和伪麻黄碱的含量测定方法。 关键词：高效毛细管电泳法 麻黄 麻杏石甘汤 麻黄碱 伪麻黄碱 Determination of Main component in Ephedra and Maxingshigan Decoction by Capillary Zone Electrophoresis Abstract: Objective To establish a method for the determination of ephedra ephedrine and pseudoephedrine in ephedra and maxingshigan decoction by capillary zone electrophoresis.Method The conditions of the experiment were optimized with a fused-silica capillary of 60 cm×50 μm i.d. (50 cm effective length ) in a running of 50 mmol/L borax-20 mmol/L threonine (pH9.27) with an applied voltage of 15 kV(room temperature). Sample introduction was performed by hydrodynamic injections(10 cm ×20 s) and determined with on-column UV monitoring at 210 nm. Phenobarbital was chosen as the internal standard. Ephedrine and pseudoephedrine are separated successfully within 8 min. Results The linear responses covered the ranges from 21.3 to 213 μg/mL(r=0.9996) for ephedrine and from 8.4 to 84 μg/mL(r=0.9995) for pseudoephedrine. The detection limits(S/N=3) of ephedrine and pseudoephedrine were shown to be 1.45 μg/mL and 1.48 μg/mL, respectively, The quantitation limits (S/N =10)of ephedrine and pseudoephedrine were shown to be 4.81 μg/mL and 4.93 μg/mL, respectively. The average recoveries for ephedrine and pseudoephedrine were 97.5% and 98.6% with RSD less than 5.0%. Conclusion The method is simple, rapid, cost-effective and precise with satisfactory results. Key words: capillary electrophoresis; ephedra; Maxingshigan Decoction; ephedrine; pseudoephedrine 麻黄为麻黄科植物草麻黄的干燥草质茎，始载于《神农本草经》。临床上治疗呼吸道疾患，具有发汗散寒、宣肺平喘、利水消肿的功效麻杏石甘汤出自《