糖尿病的文章.PPTVIP

糖尿病的文章 四英語一A 63號 郭稦玲 he fasting blood glucose concentration was determined using MediSense Card Blood Glucose Testing Systems , which uses an electrochemical detection technique with a glucose oxidase method. One day before the fasting blood glucose levels were measured, the postprandial urinary levels of ketone bodies, glucose and protein, the pH values, and the presence of blood were determined using multiple test strips . Complete blood counts, including the levels of red blood cells, white blood cells, hemoglobin, hematocrit, and platelet, were measured using a hematology analyzer . Serum concentrations of albumin, cholesterol, triglyceride, creatinine, blood urea nitrogen (BUN), and uric acid were measured by an automatic analyzer . Serum concentrations of free fatty acid and fructosamine were determined by enzymatic colorimetric assays , and the insulin concentrations in the serum and pancreas were measured by commercially available enzyme-linked immunosorbant assays . The pancreatic insulin was extracted by hydrochloride-acidified ethanol, as described by Masiello et al, prior to measuring the insulin. All the samples were analyzed in one assay in duplicate with intra-assay coefficients of variation below 10%. Animal carcass compositions, such as percentage and amount of water, protein, and fat, were measured with methods as previously described . In brief, total carcass water was determined by freeze-drying the carcasses for 2 weeks to obtain a dried carcass weight and then subtracting the dried carcass weight from the total carcass weight. The freeze-dried carcasses were then homogenized with liquid nitrogen, and aliquots of dried rat homogenate were assayed for nitrogen and fat concentrations. The carcass fat concentration was determined by extracting dried rat homogenate with ether, and then evaporating the ether to determine the fat amount. In addition, carcass nitrogen concentration was determined by micro-Kjeldahl analysis. 空腹血糖值是用MediSense卡片血糖測試系統