青枯雷尔氏菌脂肪酸型和致病性的关系.docVIP

青枯雷尔氏菌脂肪酸型与致病性的关系 刘波蓝江林车建美林营志王秋红朱育菁 （福建省农业科学院农业生物资源研究所）atty Acide Type of Ralstonia solanacearum Strains And the relationship with pathogenicity LIU Bo, LAN Jiang-lin, CHE Jian-mei, LIN Ying-zhi, WANG Qiu-hong, ZHU Yu-jing (Agricultural Bioresource Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003) Abstract: 【Objective】 Based on the analysis of relationship between the fatty acide and pathogenicity of Ralstonia solanacearum. 【Method】 The fatty acide types were divided into 3 groups, FAT , Ⅱ, Ⅲ. FAT Ⅰ was characterized with＞0.8 avirulent index, 0% infected mortality belonging to the non-pathogeniciy type, FAT Ⅱ with 0.6-0.8 avirulent index, 6.7%-100% infected mortality, belonging to Transition-virulent type in which the pathogeniciy was changeable to cause high and low mortalities to the tomato plantlets, FAT Ⅲ with ＜0.6 avirlent index, 100% stable infected mortality. 【Result】 Using cluster analysis to group the fatty acides, principle component analysis to select key factors of fatty acide, discrimination analysis to build up the discrimination model, three fatty acides of 14:0(X1), 17:0(X9), 18:0(X13) were involved to the model set as follow: Y1=-16.3353 + 0.0012X1 + 0.0056X9 - 0.0016X13，Y2=-3.4928 + 0.0002X1 + 0.0003X9 + 0.0025X13，Y3= -9.1550 - 0.0003X1 + 0.0039X9 + 0.0042X13. 【Conclusion】 The accurate rate for discrimination was up to 90.00%. The model of fatty acide type for R. solanacearum was built up to provide the experience for computer-based standardizing analysis for the pathogen evolution under species level, further more with the new idea for other pathogen differentiation. Key words: Ralstonia solanacearum; fatty acide type; pathogenicity 0 引言 【研究意义】植物细菌性青枯病是一种由青枯雷尔氏菌（Ralstonia solanacearum）引起的毁灭性土传病害。青枯雷尔氏菌可危害44个科300多种植物，造成农林业生产巨大经济损失[1]。【前人研究进展】青枯雷尔氏菌在寄主范围、地理分布、致病特征、流行特征、种下分化等方面存在较大的差异，具有明显的多态性[2]。青枯雷尔氏菌的种下分化复杂，前人从生理小种race）[3]、生化型biovar）[4]、血清型serotype）[5]、溶源型lysotype）[6]、基因型genetype）[7]等方面对种下分化进行研究。然而，许多研究表明，采用的种下分化的分析方