及酒精性肠粘膜损伤药物干预课件.pptVIP

药物 吲哚美辛（INN名称：Indometacin 氟比洛芬 flurbiprofen; 双氯芬酸 diclofenac Celecoxib mucosa injury塞来昔布 alcohol酒精 rabeprazole雷贝拉唑 Rebamipide瑞巴派特 misoprostol米索前列醇 glutamine谷氨酰胺 The gastric ulcerogenic response was examined as described previously [25], with some modifications. Mice fasted for 18 h were intravenously administered indomethacin in PBS via the tail vein and 1 h later, orally administered celecoxib in 1% methylcel- lulose in a volume of 10 ml/kg. In some experiments, mice were orally administered rebamipide in 0.5% carboxymethylcellulose in a volume of 10 ml/kg 1 h before the administration of indometha- cin. Eight hours after the administration of celecoxib, the animals were sacrificed, after which their stomachs were removed and the areas of the gastric mucosal lesions were measured by an observer unaware of the treatment they had received. Calculation of the scores involved measuring the area of all the lesions in square millimetres and summing the values to give an overall gastric lesion index. The gastric PGE 2 level was determined by EIA according to the manufacturer’s instructions. celecoxib (10 mg/kg) The effective daily dose of celecoxib in rats is 5–30 mg/kg body weight (Herbenick et al., 2008). In a similar study design, other workers have shown that the lowest dose of celecoxib that could inhibit PGE 2 in gastric mucosa was 30 mg/kg body weight (Berenguer et al., 2004). Therefore, in the present study a dose of 30 mg/kg body weight of celecoxib was selected to evaluate its effect on gastric mucosa. Experimental time of 6 h was selected as it has been shown that damage induced by the assayed drugs peak 6 h after oral administration (Villegas et al., 2002; Sanchez et al., 2002). in 0.1% methylcellulose (MC) solution, and in 0.5% MC solution for rats. celecoxib (Pfizer), 30 and 100 mg/kg comparablevolume(1mL/100g bodyweight) Induction of intestinal damage Enteritis was induced in separate groups (n = 8 rats for each group) of unfasted rats, by means of