实时荧光定量PCR在临床医学中的应用PPT课件.pptVIP

* Gene ScanningHigh-Resolution Melting Analysis In this example, the “perfect” result of a Gene Scanning experiment is depicted. In the target LPLH3, a SNP from G into T results in such a destabilization of the amplicon, that both homoduplexes (wildtype and mutant) can be discriminated as well as the heterozygous types. 目标: MBL2 384个样本中筛选突变体 复制子大小 219 bp 共发现序列不同的4个常见组和2个罕见组 文献中共描述了3个多态性位点，频率最高的等位型为以下4种： A) C / G / G B) C / A / G C) C / G / A D) T / G / G 甘露糖结合凝集素(MBL)是先天性免疫系统的重要成分之一,在免疫防御中发挥多重作用。MBL基因多态性与多种疾病易感性、严重程度以及预后密切相关。在免疫系统尚未成熟的儿童,或同时存在原发或继发免疫缺陷的患者,低表达MBL基因型与感染性疾病、自身免疫性疾病的相