母种分离技术（Mother seed separation technology）.docVIP

母种分离技术（Mother seed separation technology） In nature, edible fungi do not exist alone, but live together with many bacteria, radiation bacteria, molds and so on. The so-called separation of bacteria is to separate these edible fungi living with the bacteria, and to obtain pure and excellent strains through culture. Spawn, stock, seed and cultivated species. Seed production procedures refer to figure 13. (1) isolation and culture of mother species Separation of mother edible fungus, can be divided into spore separation, tissue isolation method and hypha separation etc.. 1. spore isolation method Spore isolation method is the method of culturing mycelium by using sexual spores or asexual spores of edible fungi. This strain has strong viability, but there are differences between individual spores, and natural differentiation phenomenon is serious, variability is large, need to be fruiting test in order to be applied in production. (L) single spore isolation: the method of obtaining only one spore per time or in each test tube and allowing it to germinate into mycelium to obtain pure strains. By single spore isolated from the mycelium of mushroom and mushroom, have strong ability, this method can be used to produce pure strains isolated. Single spore isolation is seldom used in production, and the technology is complex. (2) multi spore isolation: a method of obtaining a pure strain of edible fungi by inoculating many spores on the same medium, allowing them to germinate and freely mate. Specific methods of operation, there are the following: A mushroom spore ejection method: individual robust, round shaped flower, no pests, fruiting uniform, high yield, strong adaptability of the mushroom, eight or nine mature cut parts, stipe gauze or cotton, wash water and dry with filter paper surface sterilized several times with sterile water. In the indoor inoculation box or the kind of sterile, mushroom gills down with wire hanging upside down in a glass funnel below the funnel in