(食品质量与安全研讨会)In vitro effects of anti-oxidants from fruts and calyxes of Physalis pubescens L. on hepatocellular carcinoma cell line Malhavu.pptVIP

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2018-06-17 发布于浙江
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(食品质量与安全研讨会)In vitro effects of anti-oxidants from fruts and calyxes of Physalis pubescens L. on hepatocellular carcinoma cell line Malhavu.ppt

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(食品质量与安全研讨会)In vitro effects of anti-oxidants from fruts and calyxes of Physalis pubescens L. on hepatocellular carcinoma cell line Malhavu

In vitro effects of anti-oxidants from fruits and calyxes of Physalis pubescens L. on hepatocellular carcinoma cell line Malhavu Wang jingjing Liaoning medical university Laboratory studies have shown that various therapeutic effects are generally involved in plants of Physalis such as: antibacterial, anti-inflammatory, anticancer, immune adjustment and regulation of blood lipid level effects. objective This study aimed to identify whether, or not, antioxidants rich in phenols and flavonoids from fruits and calyxes of P. pubescens can be a candidate for the further development of an anti-hepatoma fraction, and if such biological effects coupled with reactive oxygen species (ROS) changes, can provide a direction for subsequent biological assay. My?presentation?will?cover?the?followingaspects: Preparation of tested compounds Determination of flavonoid and phenolic content Cell culture Cell proliferation assay Induced-apoptotic property assay Measurement of ROS in Malhavu cells exposed to FTPF or CTPF Assessment of antioxidant activity of FTPF or CTPF Preparation of tested compounds Cell culture——Hepatocellular carcinoma cell line Malhavu（ Mal. ） Fig. 1. Effects of TPF from P. pubescens on cell viability. Fig. 3. Cell apoptosis determined by Annexin V-FITC/PI staining Fig. 5. A, B, C, D Anti-oxidative assessment of CTFP or FTPF against different free radicals. Discussion Dual action of CTPF in different condition Failure to anti-hepatoma effect of anti-oxidative FTPF——antioxidant may be inefficient for patients with cancer Additionally, the cellular assessment is appropriate to reveal anti-oxidation of different compounds due to the close of physiological dose. Conclusions The data in this paper demonstrate the biological responses of TPF from P. pubescens upon hepatoma cell line Malhavu accompanied with pro- and reduction of ROS. Anti-oxidative CTPF is a well bioactive compound deserving further separation to the development of anti-hepat