甲状腺素诱导小鼠模型表观指征变化与阴虚火旺证的相关性的研究.doc

甲状腺诱导小鼠表观指征变化研究贺玉伟，柴程芝，寇俊萍，戚进，余伯阳（中国药科大学中药复方研究室 南京 211198） 摘要目的甲状腺素小鼠的，为阴虚火旺证动物模型构建提供参考。方法将48只标重小鼠随机分为空白组和组每组12只，雌雄各半，i.g.不同剂量（40 mg/kg、80 mg/kg、160 mg/kg甲状腺混悬液，i.g.双蒸水，并测定小鼠体重、摄食量、饮水量、体温、自发活动和心率的动态变化。结果与空白组相比较，小鼠体重增加，摄食量与饮水量，体温升高，心率加快，自主活动频繁等，能够模拟阴虚火旺证的主要临床指征，其中雌性小鼠表观指征变化更显著，与空白对照组比较有显著差异（）。结论甲状腺素造模诱导小鼠表观指征的变化模拟阴虚火旺证的主要临床特征i.g. 160 mg/kg/d连续14天诱导模型小鼠表观指征的变化与阴虚火旺证的相关性更为显著。 关键词：甲状腺素；阴虚火旺；模型Correlative Study between the Exterior Signs of Mice Model Induced by Thyroxine and Hyperactivity of Fire due to Yin Deficiency Syndrome HE Yu-wei, CHAI Cheng-zhi, KOU Jun-ping, QI Jin, YU Bo-yang State Key Laboratory of Natural Medicines, Department of Complex Prescription of TCM, China Pharmaceutical University, Nanjing, 211198, China; Abstract: Objective To observe the correlation between the exterior signs of mice model induced by thyroxine and hyperactivity of fire due to yin deficiency syndrome to provide reference for constructing hyperactivity of fire due to yin deficiency syndrome animal model. Methods 48 standard weight mice were randomly divided into control group and three model groups, 12 each group, male and female in half. Model groups were managed with thyroid tablet suspension of different doses (40 mg/kg, 80 mg/kg and 160 mg/kg) respectively, whereas the control group with double-distilled water. The body weight, food intake, water intake, body temperature, spontaneous activity and heart rate were measured dynamically. Results As compared with the control group, mice in the model groups manifested slowly increased body weight, enhanced food intake and water intake, elevated body temperature, quicken heart rate and frequent spontaneous activity, which could simulate the major clinical characteristics of hyperactivity of fire due to yin deficiency syndrome, such as emaciation, voracious appetite, thirsty for water, intermal heat, palpitation, impatience, et al. Among which, the exterior signs of female mice in high-dose model group were extremely notable compared