《黑胶虫污染》ppt课件.pptxVIP

Got black swimming dots in your cell culture? Identification of Achromobacter as a novel cell culture contaminant;abstract;;Unknown bacterial contamination can be transient. Contami- nation is common with poor aseptic technique and can be devas- tating in a research setting [3]. While many undesired organisms may steal nutrients from cell lines in culture, they may also prey on the cells themselves. Predatory bacteria have been shown to feed on other bacteria [5,6], particularly in a limited nutrient environ- ment[7]. Experimental results mayalso be altered due to unwanted activation of cells. Different cellular functions, including those triggered by Toll-like receptors, can be activated by a variety of bacterial components. Several online science blogs discuss a cell culture contaminate that looks like black specks in the cell culture (http://www. /i.html/T3470/H3448.html; to /forums/index.php?s?bc4fecd655ee6898191ab6ed 43f5a5a7showtopic?9373pid?30730st?0). The researchers on these blogs describe black dots that fidget or swim and look more like rods than dots. Our laboratory has experienced sporadic cell culture contamination fitting this description. Here we report the identification of a bacterial cell culture contaminate, a member of the Achromobacter genus, matching anecdotal descriptions of cell culture contaminants of multiple eukaryotic cell lines. In addition, we provide information regarding antibiotic resistance and treatment effectiveness.;Antibiotics and doses tested to treat Achromobacter contaminated cells. Antibiotic Dose ( m g/ml) Gentamicin庆大500–2000 Amikacin 50–2500 Tobramycin妥布霉素50–600 Imipenem 2–64 Erythromycin 25–500 Piperacillin哌拉西林0.5–1000 Choramphenicol氯霉素50–500 Ciprofloxacin环丙沙星10–750 Plasmocin 25–62.5清除支原体污染;;;Initial attempts to lyse裂解the culture contamination were done in the eukaryotic cell culture supernatant. Boiling (5–10 min at 95 .C)and sonicating声波降解标本(15–30 s at output power 4) did not result in available DNA for PCR. It is po