RNA沉默的过去现在与将来1.pptxVIP

农学与生物技术学院 马明慧 2011210095; RNA silencing is to point to in eukaryotes (plants, animals, fungi) by double-stranded RNA conservative induction identification and destroy abnormal variation in the cytoplasm of RNA expression or a mechanism.;RNA silencing first observed in plants during transgene studies;;First demonstrated to be RNA-mediated during pathogen-derived virus resistance studies in plants; In this article, we review the rich history of gene silencing research and the knowledge it has generated into our understanding of this fundamental mechanism of gene regulation in plants; describe examples of the current applications of RNA silencing in crop plants; and discuss improvements in RNA silencing technology and its potential application in plant science.;;Both relation and difference;;Both relation and difference; MiRNA pathway miRNA is content rich not coding small RNA (21 ~ 24 nucleotide), Dicer enzyme expression by cutting short clip structure of endogenous RNA) form. MiRNA can also and albumen factor formation RISC protein complex, can be combined and cutting specific mRNA and cause RNA silence.;Current model of RNA silencing;; Although trigger silent different sources, but siRNA and miRNA all involved in a structure similar RISC, in effect both on way has a lot of similarities. ;Next welcome to my partner！;农学与生物技术学院 郭圣烨 2011210092;;RNA silence fall into two types of approach;RNAi can be used to protect a plant against invading or attacking organisms other than viruses? ; Agrobacterium is a soilborne bacterium that causes crown gall disease, which imposes signi?cant economic losses in perennial crops worldwide. It has a horizontal gene transfer system for a suite of oncogenes that, when integrated into the plant genome, generates tumor formation. ;; Escobar and colleagues (2001) tested whether RNAi could be used to control this plant parasitic pathogen. They transformed tomatoes with hpRNA constructs against the iaaM and ipt oncogenes,