The 3rd Rsearch on Theorem Proving第三论定理证明.pptVIP

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2018-06-19 发布于福建
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The 3rd Rsearch on Theorem Proving第三论定理证明.ppt

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The 3rd Rsearch on Theorem Proving第三论定理证明

Integrated Microsystem of Isothermal Amplification of DNA and Electrophoresis on a Microfabricated Plastic Chip for Detection of Specific Gene and Analysis of Genetic Materials MEC Seminar Proteome Research Lab 2003. 5. 20 Summarized by Park, Ji-Yoon Continuous-Flow PCR on Chip Science, 280, 1046-1048(1998) Infrared-mediated Thermocycling for DNA Amplification and Electrophoretic Separation on an Integrated Microrchip DeviceProceedings of the uTAS 2002 Symposium, Vol. 1, pp 193-194 DNA Amplification and Hybridization Assays in Integrated Plastic Monolithic DevicesProceedings of the uTAS 2002 Symposium, Vol. 1, pp 169-171 On-chip integration of isothermal PCR and electrophoresis Loop-mediated isothermal amplification(LAMP) Novel DNA amplification method developed by Eiken Chemical Co., Ltd. By using 4 different primers specifically designed to recognize six distinct regions on the target gene Performed at a constant temperature using a strand displacement reaction. Advantages High amplification efficiency( 109-1010 times in 15-60 minutes) High specificity The potential to replace PCR ? simplicity, rapidity, specificity and cost-effectiveness Avoid the thermal cycling Analysis by the integrated microchip electrophoresis by the naked eyes by adding fluorescent dye like SYBR Green I Loop-mediated Isothermal Amplification (LAMP) RAM(Ramplification Amplification Method) Chip Device and Electropherogram Integrated microchip for DNA amplification and CE Reaction condition RAMP; 5- 10 ul volume, 50-65°C for 10-30min Micro-CE - Reproducibility(Fig. 4) - Detection time; 10min (Fig 5) - Visualization(Fig 6) SYBR Green? I The dye (ex; EtBr) Binds preferentially to ds DNA quantitate the amount of double-stranded product Stable to the extremes of temperature required for PCR reaction The Reproducibility and Detection Conclusions Integrated microsystem Useful for fast, sensitive, and simple detection o