What is Fluorescnce Coil线圈是荧光.ppt

* Dyes and stains used to detect structures and processes in biological samples. The use of fluorescent molecules has revolutionalized microscopy and here I will give you an introduction as to how these molecules work. The kinds of fluorescent molecules that are available and a brief overview of some more advanced fluoresecent techniques. * * Light is a form of energy known as electromagnetic radiation. The energy is contained in units or quanta called photons that have the property of both particles and waves. Helpful to think of light as a particle in some microscopy applications, such as the quantitative detection of light as it falls on a measuring device such as a CCD camera. But in terms of fluorescence it is usually most relevant to think of light as a wave. The usefulness of light in microscopy depends on its wavelength. It is important to note that the light we perceive as visible occupies only a very small part of the electromagnetic spectrum. * * * * * * * * * * * * * * * * * * * * * * * * * * * * How fluorescence works Adele Marston Topics covered The nature of light and colour Colour detection in the human eye The physical basis of fluorescence Fluorescent probes and dyes Dyes that bind organelles Chemical Dyes Fluorescent proteins Photobleaching and Quenching The Nature of Light The energy of light is contained in discrete units or quanta known as photons Light is a form of electromagnetic radiation Photons have the property of both particles and waves For simplicity, usually only the electrical component is drawn Light as a wave: The nature of light and colour - 1 The Electromagnetic Spectrum Wavelengths 400nm-750nm are visible to the human eye The nature of light and colour - 2 The Human Eye Sensitivity Peak sensitivity is at 555nm (yellow-green) In bright light, 3 orders of magnitude After time to accommodate, 10 orders of magnitude! Resolution ~0.1mm for an object 25mm from the eye Composed of Rod and Cone cells Can detect dif