提取IgG的具体步骤-英文.docVIP

Purification of Antiserum or Ascites by Protein A/G Chromatography Required Materials and Equipment Protein A or G agarose gel column (10 ml or 5 ml of packed beads; see guidelines below for choice of protein A or protein G) Ice-cold Tris-buffered saline (TBS). See recipe below. 5% sodium azide solution Neutralization Buffer (NB). See recipe below. Elution Buffers (pH 2.7 and pH 1.9). See recipes below. Centrifuge tubes and microcentrifuge tubes Preparative centrifuge and microcentrifuge pH strips Microtiter plate reader with 600 nm filter Glass Columns Guidelines for Choosing Protei