舒糖宝与荔枝水提取液对糖尿病小鼠模型疗效比较研究.docVIP

舒糖宝与荔枝水提取液对糖尿病小鼠模型疗效比较研究 　　[摘要] 目的 探讨中药舒糖宝与荔枝水提取液对糖尿病小鼠模型的治疗效果。 方法 用ALX与 STZ造模小鼠，并分成模型组、治疗1组及治疗2组，另设正常组。治疗1组采用舒糖宝治疗，治疗2组采用荔枝提取液治疗， 模型组和正常组不采取任何治疗。测定造模前后及治疗后血糖，并测定治疗结束时血清生化指标。 结果 造模前各组血糖差异无统计学意义（P0.05）；造模后三组血糖与正常组比较，差异均有统计学意义（P 　　[关键词] 糖尿病；血糖；中药 　　[中图分类号] R969.4 [文献标识码] A [文章编号] 1674-4721（2013）11（c）-0011-03 　　Comparison study of the curative effects of shutangbao and litchi water extract on diabetic mouse model 　　GUO Hai-yan HUANG Bing-chen HUANG Li-ping HUANG Jie-lin LI Pei-lei WANG Ya-wei ZHANG Shu-qiu HE Sheng 　　Heavy Metals and Fluorine-arsenic Toxicology Research Laboratory of Youjiang Medical College for Nationalities，Baise 533000，China 　　[Abstract] Objective To explore the curative effect of Chinese medicine shutangbao and litchi water extract on diabetic mouse model. Methods ALX and STZ were used to establish the models of diabetic mouse，then they were divided into the model group，treatment group 1，treatment group 2 and the normal group.Treatment group 1hey was treated with shutangbao，treatment group 2 was treated with litchi water extract，no treatment on the model group and the normal group.The blood glucose of mice were measured before and after the models set up，and the blood biochemical indicators were measured at the end of treatment. Results Before the models set up，the differences of blood glucose content between the groups were not statistically significant（P0.05）；the differences of blood glucose content between the normal group and other groups were statistically significant（P 　　1 材料与方法 　　1.1 仪器与试药 　　ALX、STZ均为Sigma公司产品；枸橼酸、枸橼酸钠，硫脲、硼酸、邻甲苯胺、冰乙酸、标准葡萄糖均为国产分析钝。总胆固醇（TC）、三酰甘油（TG）、尿素测定试剂盒购自四川成都迈克试剂公司和南京建成生物工程研究所。舒糖宝采用酸荔枝、番石榴、沙田柚等果类提取制成，由本院海尔福研制中心提供。荔枝肉提取液为本市靖西县产的酸荔???，肉味酸，核大，属古老品种，生长于山坡上，取其肉，60℃烤干（8 h），称重后，水提取制得提取液0.1 g/ml。 　　1.2 实验动物 　　90只健康小白鼠，昆明种，雌雄各半，其中12只鼠龄2个月，体重20～25 g，其余为鼠龄3个月，体重30～40 g，由本校动物室提供（动物使用许可证：syxk桂2011-0010；动物生产许可证号：scxk桂2012-0003）。 　　1.3 实验方法 　　选取78只小白鼠（12只鼠龄2个月，其余为鼠龄3个月）造模，先用ALX（Sigma公司生产，453116/140204175），剂量按每日220 mg/kg体重标准