菊苣水提取物干预高嘌呤饮食you导的鹌鹑尿酸及脂代谢紊乱的实验研究.docVIP

　　菊苣水提取物干预高嘌呤饮食诱导的鹌鹑尿酸及脂代谢紊乱的实验研究 作者：杨红莲,张冰,刘小青,林志健 【摘要】 目的 观察菊苣水提取物对高嘌呤饮食诱导的鹌鹑尿酸(UA)、脂代谢紊乱的影响,并探讨其作用机制。方法 鹌鹑按体重随机分为5组：正常组、模型组、阳性药组及菊苣水提取物大、小剂量组,每组15只。正常组给予普通饲料,其余各组均予以造模饲料。阳性药组予苯溴马隆按20 mg/(kg·d)灌胃,菊苣水提取物大、小剂量组分别予15、l0 g/(kg·d)灌胃,正常组与模型组予等体积蒸馏水灌胃,每日1次。7 d后检测UA、三酰甘油(TG)、游离脂肪酸(FFA)、总胆固醇(TC)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)水平及尿酸代谢相关酶黄嘌呤氧化酶(XOD)、腺苷脱氨酶(ADA)、鸟嘌呤脱氨酶(GD)活性。结果 与正常组比较,模型组UA、TG、FFA显著升高,HDL显著降低；与模型组比较,菊苣水提取物大、小剂量组可显著降低血UA、TG、FFA水平。模型组GD活性较正常组显著升高,菊苣水提取物大、小剂量组可显著降低GD活性；各组XOD与ADA活性比较差异无统计学意义。结论 菊苣水提取物具有明显的调节尿酸、脂代谢紊乱的作用,可能的机理是通过降低GD活性而降低UA水平,通过降低UA、FFA水平从而降低TG水平。 【关键词】 菊苣；高嘌呤饮食；尿酸；脂代谢；鹌鹑 Abstract：Objective To observe the influence of Cichorii extract on uric acid and lipid metabolic disorder and discuss the mechanism. Methods Quails were divided randomly into 5 groups by weight：normal group, model group, positive control group and Cichorii extract high and low dosage group, 15 quails in each group. The normal group was fed with the common feeder, and the other groups were fed with the yeast powder. Positive control group was given Benzbromarone 20 mg/(kg·d). Cichorii extract high and low dosage group were given Cichorii extract 15, 10 g/(kg·d) respectively. Normal and model group were given distilled water. UA, TG, FFA, TC, HDL, LDL level and XOD, ADA, GD activity related to uric acid metabolism were determined after 7 days. Results Compared with normal group, UA, TG, FFA of model group was significantly higher and HDL was significantly lower. Compared with model, Cichorii extract decreased UA, TG, FFA significantly. GD activity in model group increased significantly. Cichorii extract decreased GD activity. There was no significant variance of XOD and ADA activity in each group. Conclusion Cichorii extract had obvious effect in adjusting uric acid and lipid metabolic disorder. The possible mechanism was by decreasing GD activity and decreasing UA, FFA level. Key words：Cichorii extract；high-purine diet；uric acid；lipid metabolism；quail 菊苣出自《新疆中草药手册》,系维吾尔族习用药材,为菊科植物菊苣Cic