Final Design Prototype - CAE Users最终的设计原型CAE用户.pptVIP

Team Cherry Picker Blake Hondl Amit Mehta Jon Millin Ryan Pope Team Small Molecule Blake Hondl Amit Mehta Jon Millin Ryan Pope Client Advisor No?l R. Peters, M.S. Keck-UWCCC Small Molecule Screening Facility Abstract High throughput screening (HTS) involves secondary testing of chemicals that initially show desired characteristics Done manually and prone to errors Goal is to design a device to guide a micropipette user to a single microtiter well The device will increase efficiency and reduce user error Device implementations Java Application LCD Screen User Interface Problem Statement Design a device to guide a micropipette user in the transfer of small volumes of compounds between 384-well microtiter plates The device will increase the efficiency of transfers and reduce user error Background High Throughput Screening Used in cancer and RNA research Secondary testing performed on the chemicals that initially show promise Use of plate reader to determine potential “hits” Motivation Secondary testing is typically done manually, making it difficult and prone to errors Row and column identification is necessary Interior wells are difficult to locate Errors lead to increased time costs Only step in the process that is prone to significant human error Current Competition Matrix Memowell? 96 well pipetting aid Lights up 4 wells when used with 384-well plate Costs approx. $1000 Quadra Cherry Picker Automated Costs $150,000 Design Constraints Must interface with Microsoft? Excel? and user Effectively guide the user to the appropriate wells Minimize required bench space Dimensions smaller than 8.5” x 11” Withstand exposure to various chemicals and reagents Dimethyl Sulfoxide Lightweight (10 lbs) and inexpensive ($1000) Final Design Java Application User input to locate wells in one or two plates Excel input from spreadsheet (future work) LCD Screen to show output from Java Application Lines (cross-hairs) displayed to identify wells to user Final Design Protecti