七科联考免疫学学免疫学 补体Compemen.ppt

细胞膜上的调节因子 膜辅蛋白：MCP 抑制MAC形成： 衰变加速因子（DAF） C8结合蛋白 CD59 (膜反应性溶血抑制剂） 四、补体的生物学作用 溶菌杀菌细胞毒作用：MAC 免疫调理作用：C3b，C4b 免疫复合物清除：C3b，C4b 炎症介质： 过敏毒素 (C3a C4a C5a) 趋化作用 (C3a C5a C567) 激肽样活性 (C2a C4a) Opsonization and phagocytosis 思考题 细胞培养所用的血清为什么需要 56度30分钟灭活？ * * * * * * * * Classical Pathway Generation of C3-convertase C4b Mg++ C4a Ca++ C1r C1s C1q C2 C2a b C2 b C4b2b is C3 convertase Classical Pathway Generation of C5-convertase C4b Mg++ C4a Ca++ C1r C1s C1q C2a C2 b C3 C3a b C4b2b3b is C5 convertase; it leads into the Membrane Attack Pathway C4b C2b C3b C5-convertase of the Classical and lectin Pathways Components of the lytic pathway C6 C 9 C8 C7 C5 Lytic pathwayC5-activation C3b C2 b C4b C5 b C5a Lytic pathwayassembly of the lytic complex C5 b C6 C7 Lytic pathway:insertion of lytic complex into cell membrane C5 b C6 C7 C8 C 9 C 9 C 9 C 9 C 9 C 9 C 9 C 9 C 9 C3 转化酶形成 C3 正反馈循环 C5 转化酶形成 攻膜复合体形成 补体激活的旁路途径 Components of thealternative pathway C3 B D P 旁路途径 C3 C3b C3bB B D Ba C3bBb P(备解素） C3bBb3b C3转化酶 C5 转化酶 Spontaneous C3 activation C3 H2O i B D C3 C3a b Generation of C3 convertase This C3b molecule has a very short half life b Components of thealternative pathway C3 B D P B D b C3b If spontaneously-generated C3b is not degraded C3-activationthe amplification loop C3 C3a b C3a B D Bb C3b C3 b C3-activationthe amplification loop C3b C3a b C3a C3a Bb C3b C3b C3 Bb B D b b C3a C3-activationthe amplification loop C3b C3a C3a Bb C3b Bb Bb C3b C3a C3-activationthe amplification loop C3b C3b C3a C3a Bb C3b Bb Bb C3a C3-activationthe amplification loop C3b C3b C3b stabilization andC5 activation C3b C3b finds an activator (protector) membrane C3 C3a b C5 b C5a B D b P This leads to membrane attack pathway C3b Bb C3b C5-convertase of the Alternative Pathway Lytic pathway:insertion of lytic complex