Beyond Fluorescence Line Height – Making sense of - Ocean Color除了荧光线高度–了解海洋的颜色.pptVIP

Beyond Fluorescence Line Height – Making sense of - Ocean Color除了荧光线高度–了解海洋的颜色.ppt

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Beyond Fluorescence Line Height – Making sense of - Ocean Color除了荧光线高度–了解海洋的颜色

Beyond Fluorescence Line Height – Making sense of chlorophyll fluorescence Ru Morrison (Ru.Morrison@unh.edu) Mike Behrenfeld (behrenfm@science.oregonstate.edu) You can’t see a whale from space but you can see … Outline What is phytoplankton fluorescence? The current fluorescence paradox Fluorescence variability Games of chance Changing the odds – 2003, 2008, 2009 What fluorescence can do What fluorescence can’t do What we need to do! What is phytoplankton fluorescence? The Fluorescence Paradox Two potential and mutually incompatible uses Abundance - need constant fluorescence efficiency Physiology - need variable fluorescence efficiency. Fluorescence variability – games of chance Fluorescence comes from PSII (mainly) Φf = chance getting an absorbed photon to PSII and then back out again Variability comes from quenching Photochemical quenching (qP) Non-photochemical quenching Antennae quenching (qE) PSII RC downregulation / damage (qI) Changes in proportion of PSII to total absorption Changing the odds - 2003 Changing the odds – 2008 Nutrient stress Detection of iron stress effects. Seasonal Climatology Timing of stratification onset and spring bloom What can Fluorescence do Give insights into phytoplankton physiology Iron stress Photocompensation / acclimation with growth irradiance that incorporates hydrodynamics What Fluorescence can’t do Abundance apart from geographically and spatially limited studies. What do we need to do? Minimize calibration uncertainties in the FLH product Develop operational φf product Investigate the heck out of it in-light of these recent insights. With current missions How do you separate nutrient and photoacclimation effects? For upcoming missions (GEOCAPE interesting) OCRT 5/5/2009 JRM MB Fluorescence Seeing inside phytoplankton from space Fluorescence Line Height Fluorescence Efficiency or quantum yield, φf ratio of photons out to in NPQ Dominates in Satellite Derived measurements Energy Dependent Quenching – decreases φf with

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