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Investigations into the epidemiology of Campylobacter isolated from sheep and cattle: food safety management aspects Anna Garcia Lecturer in Veterinary Public Health Campylobacter sheep Results (38%-60%) (53%-73%) (88%-98%) (81%-95%) Campylobacter spp. sheep C.coli 16% Other 5% NO ID 4% C.jejuni 75% Campylobacter sheep Risk Factors Final logistic regression model showing the most significant variables for the presence of Campylobacter on the carcases. ? Coef. SE OR 95% CI p-value ? Cleanliness 2.33 0.89 10.24 1.77-59.18 0.009 (acceptable but not very clean) ? C. jejuni 2.20 1.13 9.00 0.97-83.46 0.053 positive faecal sample Final logistic regression model showing the most significant variables for the presence of Campylobacter jejuni on the carcases. ?  Coef. SE OR 95% CI p-value ? Time of the year 3.89 1.22 49.03 4.44-541.14 0.001 (winter) Campylobacter cattle /cgi/content/full/56/11/1467 J. Med. Microbiol., Nov 2007 C. jejuni C. coli C. fetus Campylobacter cattle study results Fd- Faecal samples direct plating Fe- Faecal samples after enrichment C- Carcases before chilling Ca-Carcases after 24 hours chilling Campylobacter ID results MLST ANALYSIS Results MLST analysis MLST performed 43 samples ST identified 15 samples MLST RESULTS (PubMLST) Fd2 C.jejuni ST 436 has been isolated from human stools (UK, 2004 Netherlands, 1998) Fd17 C.jejuni ST-97- ST-45 complex. This ST has been isolated from chickens (NZ, 1993) chicken offal/meat (UK, 1982) wild bird (UK, 2001) F86 C.jejuni- ST-52- ST-52 complex. This ST has been isolated from humans (stools and blood), chicken and sheep in UK, Netherlands, Australia, Canada and Curacao C30 C.coli 855 ST-828 complex. This ST has been isolated from chickens in UK.? C69 C.jejuni New ST?? C93 C.coli ST-1405 ST-828 complex. This ST has been isolated from chickens in UK. Quantification Campylobacter Direct plating (dilutions): Carcases Carcases after chilling Results: very small colonies