三七透明质酸钠凝胶对家兔硬膜外瘢痕中成纤维细胞凋亡的影响.docVIP

三七透明质酸钠凝胶对家兔硬膜外瘢痕中成纤维细胞凋亡的影响 ? 282?WORLDCHINESEMEDICINEJuly.2010,Vo1.5,No.4 三七透明质酸钠凝胶对家兔硬膜外瘢痕中成纤维 细胞凋亡的影响 刘灿周卫孔焕宇徐泉李莉章永东张兆杰 (中国中医科学院望京医院,北京市朝阳区花家地街,100102) 摘要目的:研究三七透明质酸钠凝胶(以下简称三七凝胶)对硬膜外瘢痕组织中成纤维细胞凋亡的影响.方法:选取大耳 白品系家兔作为实验动物,制作家兔椎板切除模型,在硬膜囊周围分别滴加三七凝胶,三七浓缩液,透明质酸钠,生理盐水,于术 后第2,4,8周取材.通过末端脱氧核糖核苷酸转移酶(TdT)介导的原位缺口末端标记法(Tune1)和原位杂交法观察各组局部 硬膜外瘢痕中成纤维细胞凋亡及半胱氨酸天冬氨酸蛋白酶一3(Caspase一3)的表达情况.结果:对硬膜外瘢痕中成纤维细胞凋 亡的Tunel染色显示,三七凝胶组在第2,4,8周的细胞凋亡指数显着高于生理盐水组和透明质酸钠组(Plt;0.01),且于第2周 与三七浓缩液组无明显差异;透明质酸钠组在第2,4,8周与生理盐水组均无明显差异(Pgt;0.05).原位杂交检测Caspase一3 表达的结果显示,实验第2周时三七凝胶组的Caspase一3表达量最高,较其他各组均具有显着性差异(Plt;0.05),三七浓缩液 组较生理盐水组高,有统计学意义(Plt;0.05);第4周时各组间均无统计学差异;第8周时三七凝胶组的Caspase一3表达量均 显着高于生理盐水,三七浓缩液及透明质酸钠组(分别Plt;0.01,0.01,0.05).结论:三七凝胶可明显诱导成纤维细胞凋亡,促 进凋亡执行因子Caspase一3的表达;透明质酸钠对瘢痕中成纤维细胞凋亡的影响不大;三七凝胶诱导瘢痕中成纤维细胞凋亡 的机制可能与促进Caspase一3的袁达有关. 关键词三七;硬膜外瘢痕/中医药疗法 EffectofSanqiSodiumHyaluronateGelOilApoptosisofFibroblastinRabbitsEpiduralScar LiuCan,ZhouWei,KongHuanyu,eta1. (Wan~ingHospital,ChinaAcademyofChineseMedicalSciences,Add.:HuajiadiStreet,ChaoyangDistrict,Beoing,Postcode: 100102) AbstractObjective:TostudyeffectofSanqisodiumhyaluronategel(thege1)onapoptosisoffibroblastinrabbitsepiduralscar. Methods:Afterlaminectomy,therabbitsweretreatedwithSanqigel,Sanqiconcentration,Sanqisodiumhyaluronateandsalinerespec— tively.At2nd,4thand8thweekafteroperation,apoptosiswasobservedbytheTunelandexpressionofCaspase-3wasdetectedbyhy— bridizationinsitu.Results:AtaJ1timepointsapoptosisofgelgroupweregreaterthanofsodiumhyaluronateandsaline(Plt;0.01),and statisticallysamewithconcentrationat2ndweek.Hyaluronatetreatedgroupisstatisticallythesamewithsalinegroup.Theexpressionof Caspase一3ingelgroupwashigherthanothergroupsat2ndweek(Plt;0.05),ofwhichconcentrationgroupWaSsignificantlyhigherthan salinegroup;ThereWasnodifferenceat4thweek;at8thweek,thegelgroupisremarkablyhigherthansalinegroup(Plt;0.01),con- centrationgroup(Plt;0.01)andhyaluronategroup(Plt;0.05).Conclusion:Sanqigelcouldinducefibroblastapoptosisthroupupregu latingofCaspase-3expression,wh