Lab 1 - Homeostasis and Diffusion - UCF Burnett School of 实验1的平衡与扩散UCF的伯内特学院.docxVIP

Lab 1: Diffusion and Homeostasis Learning objectivesExamine the effects diffusion has on animal cellsDemonstrate how tonicity is important to the human bodyExamine the effects moderate exercise has on homeostatic control mechanisms.Part I: DiffusionUnderstanding concentration gradients is essential to understand life processes. Any time a cell moves a substance across its plasmamembrane;a concentration gradient is involved in or established by the process. Dialysis tubing is used to simulate the effects of diffusion on an animal cell.This activity simulates osmosis in the cell, specific how red blood cells move water across their membrane to regulate and maintain an isotonic environment. One example of the effects tonicity has in a clinical setting is the use of an IV-Drip bag with a known concentration of solution of solutes. Depending on the situation, physicians can manipulate the solute concentration of the IV-Bag to achieve desired outcomes in treatment. It is important that you understand the concept of homeostasis and diffusion demonstrated in this experiment, because as you get further into your study of the human body, you will notice that these principles are functionally significant to each of the different organ systems.MaterialsThistle tubeBurette clampRubber bandWax pencilBeaker of unknown solutionBeaker of a known [Na+] solutionOne segment of semi permeable dialysis tubingOne bottle of de-ionized water One piece of stringSmall rulerProcedureTake the thistle tube and affix half of the dialysis tubing to end of the tube and use a rubber band to secure it.Use the piece of string to tie off the other end tightly. This is the critical part of thelab, If you do not do this correct it will leak and will have to be repeated.After securing the dialysis tubing to the thistle tube, place it on the burette clamp.Add deionized water to the thistle tube until it reaches about half way up. Place the thistle tube into a beaker filled with the known solution.Use a