外源GABA对NaCl胁迫下中间锦鸡儿的分子调控作用-Wiley.DOCVIP

Supplementary table S1. Primers for Semi-quantitative RT-PCR Clones Upstream primers（5′-3′） Downstream primers（5′-3′） CaGR1 TCATTCGGTATTGTGATGTGGG AAATCATTTGTGGACTTGGGTG CaGR2 CCTGAATCTTGTGATCCAGAATGG AAATCATTTGTGGACTTGGGTG CaGR3 CACATGCTCCTCAATTTCAACA TACAGGCTTTGGACGTGACATT CaGR17 AGGATGCTCCGAGAAGAACTG TTGGCAGAACTAAATGAAGCAC CaGR18 CAATCCAAGGGGTCTTTTGGC TTGGCAGAACTAAATGAAGCAC CaGR19 TCCCTCACAGTGGAAACTACCC AGTCCCACCTTCTTCACCAATC CaGR30 CCCAAGTAATTACCGTTATGGAGACT AGGACCCTTGTCACCGAACTTA CaGR31 ACAGATGCGGGTGGCATAAT CCATTGCTCAACACTTCAACTTG CaGR60 TGGGTTACTCGTGAGCAAGGT CTTGACCCTTGTCCCTGAAAC CaGR61 GGCGGAAGAAATCATCAAAGA ACTCCTGTCATGCTCCTGCTC CaGR62 AACCGCACAGTCACTCAACG CTCCCAGTTAGCCCATTTCAC CaGR63 CAGTCATTCCCTCATCGTAGC TATGTCAGATTGCGGAAGGAT CaGR64 AATGAAGCACAGGCTCGTTTA GCAAGTCATCAGCCATAGCAC CaGR65 CAGCTCATGGACGAGAACAAG TTGACATCTTTGCTGCGAGTT CaGR66 CCTCCATTGCGTCCCTTAGTT TGTTCTCGTCCATGAGCTGTTAT ACTIN GAGGCTCCACTCAACCCA CAGCGAGATCCAAACGAA Supplementary table S2. SSH clone of forward subtractive cDNA library Note: Blast result E-value0.001 in GenBank, the cDNA clones were considered to be have significant homology to the known sequence. SSH clone of forward subtractive cDNA library NO. Size(bp) Functions predicted Accession Signal transduction CaGR1 646 Octicosapeptide/Phox/Bem1p; ATP binding / protein kinase/ protein serine/threonine kinase/ protein-tyrosine kinase NP_190276 CaGR2 286 Octicosapeptide/Phox/Bem1p; ATP binding / protein kinase/ protein serine/threonine kinase/protein-tyrosine kinase; protein kinase family protein, similar to MAP3K delta-1 protein kinase NP_190276 CaGR3 268 Octicosapeptide/Phox/Bem1p (PB1) domain-containing protein NP_565256 CaGR4 750 NAK( NFkappaB-activating kinase ); APK2A (PROTEIN KINASE 2A); ATP binding / kinase/ protein kinase/protein serine/threonine kinase/ protein-tyrosine kinase NP_172889 CaGR5 900 NAK; APK2A; ATP binding / kinase/ protein kinase/ protein serine/threonine kinase/ protein-tyrosine kinase NP