哺乳动物自噬研究方法.docVIP

Cell Vol. 140, Issue 3, February 5, 2010 Featured Articles 特辑 Primer 引子 Methods in Mammalian Autophagy Research p313 哺乳动物自噬研究的方法 Featured Articles 特辑 Article 研究文章 Aplysia CPEB Can Form Prion-like Multimers in Sensory Neurons that Contribute to Long-Term Facilitation p421 海兔CPEB能在促进长时程易化的感觉神经元中形成阮蛋白样多聚体 Articles 研究文章 1. Acid Extrusion from Human Spermatozoa Is Mediated by Flagellar Voltage-Gated Proton Channel p327 鞭毛电压门控质子通道介导人类精子排酸的过程 2．Double-Stranded RNA-Dependent Protein Kinase Links Pathogen Sensing with Stress and Metabolic Homeostasis p338 双链RNA依赖性的蛋白激酶联接病原感知与应激和代谢稳态 3. Treslin Collaborates with TopBP1 in Triggering the Initiation of DNA Replication p349 Treslin与TopBP1共同触发DNA复制的起始过程 4．Nucleoporins Directly Stimulate Expression of Developmental and Cell-Cycle Genes Inside the Nucleoplasm p360 5．Chromatin-Bound Nuclear Pore Components Regulate Gene Expression in Higher Eukaryotes p372 6．USP10 Regulates p53 Localization and Stability by Deubiquitinating p53 p384 USP10通过将p53去泛素化调节p53的定位以及稳定性 7．RIG-I Detects Viral Genomic RNA during Negative-Strand RNA Virus Infection p397 RIG-I在负链RNA病毒感染的过程中探测病毒基因组RNA 8．Distinct Protein Domains and Expression Patterns Confer Divergent Axon Guidance Functions for Drosophila Robo Receptors p409 迥异的蛋白结构域和表达模式赋予果蝇的Robo受体以多样的轴向引导功能 Snapshot 快照 SnapShot: : Pathways of Antiviral Innate Immunity p436 SnapShot：抗病毒的自然免疫通路 Featured Articles 特辑 Primer 引子 Methods in Mammalian Autophagy Research p313 哺乳动物自噬研究的方法 Noboru Mizushima, Tamotsu Yoshimori, Beth Levine Summary: Autophagy has been implicated in many physiological and pathological processes. Accordingly, there is a growing scientific need to accurately identify, quantify, and manipulate the process of autophagy. However, as autophagy involves dynamic and complicated processes, it is often analyzed incorrectly. In this Primer, we discuss methods to monitor autophagy and to modulate autophagic activity, with a primary focus on mammalian macroautophagy. Featured Articles 特辑 Article 研究