不同倍性泥鳅misgurnus anguillicaudatus鳍细胞系的建立及特性分析-establishment and characterization of misgurnus anguillicaudatus fin cell lines with different ploidy.docxVIP

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2018-06-28 发布于上海
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不同倍性泥鳅misgurnus anguillicaudatus鳍细胞系的建立及特性分析-establishment and characterization of misgurnus anguillicaudatus fin cell lines with different ploidy.docx

不同倍性泥鳅misgurnus anguillicaudatus鳍细胞系的建立及特性分析-establishment and characterization of misgurnus anguillicaudatus fin cell lines with different ploidy

AbstractOrientalweatherfish(Misgurnusanguillicaudatus)isoneofthemostpopularculturedspeciesinChina,KoreanpeninsulaandJapanesearchipelago.Primarycultureoffinfromdiploid,triploidandtetraploidorientalweatherfishwasperformedwithtissueexplantmethod.Untilnow,cellsofthreetissueshavebeensubculturedtopassage84fordiploid,75fortriploid,and68fortetraploid,respectively.Thespecialandcombinedasepticprocessingmethodwasimmersingthefintissuein10%povidone-iodinefor15min,andtheninpenicillinandstreptomycinmixture(500IU/mLpenicillinand500μg/mLstreptomycin)for30min.ThemediumofprimarycultureandearlysubculturewasDMEM/F

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不同倍性泥鳅misgurnus anguillicaudatus鳍细胞系的建立及特性分析-establishment and characterization of misgurnus anguillicaudatus fin cell lines with different ploidy.docxVIP