不同糖耐量人群scd40l vcam-1 il-6 tnf-αmrna异常表达与血管内皮功能关系研究-relationship between abnormal expression of scd 40 l vcam - 1 il - 6 tnf - α mrna and vascular endothelial function in people with different glucose tolerance.docxVIP

RelationshipbetweenmRNAexpressionofsCD40L，VCAM-1，IL-6，TNF-alphaandendothelialfunctioninindividualswithimpairedglucosemetabolismAbstractObjective:TodetecttheexpressionofserumsolubleCD40ligand(sCD40L),high-sensitivityC-reactiveprotein(CRP),interleukin-6(IL-6),tumornecrosisfactor-α(TNF-α)andtofindtherelationshipbetweentheexpressionwithendothelialfunctioninindividualswithimpairedglucosemetabolism.Methods:90patientswithimpairedglucosemetabolismwereincludedandequallydividedasIGRgroup(IGRgroup),type2diabetesgroup(T2DMgroup),T2DMwithvascularlesionsgroup(T2DM+VCgroup)and30-machedcasesasnormalcontrolgroup(NCgroup).①sCD40L,IL-6,TNF-αmRNAexpressionlevelsinthemononuclearcellsweremeasuredbyRT-PCRmethod.②sCD40L,serumIL-6,TNF-α,VCAM-1levelsweretestedbyenzyme-linkedimmunosorbentassay(ELISA).③CRPlevelsweretestedbyimmunoturbidimetryassay.④Brachialarteryflow-mediateddilation(FMD),brachialarterynon-endotheliumdependentdilatation(NID),carotidarteryintima-mediathickness(MIT)andcardiacstructureparametersweremeasuredbyDopplerultrasound.⑤Fastingseruminsulin(FINS)contentwastestedbyalkalinephosphatase-labeledCLIA.⑥Totalcholesterol(TC),triglyceride(TG),lowdensitylipoproteincholesterol(LDL-C),highdensitylipoproteincholesterol(HDL-C)lecelsweretestedbyBeckmanLX20automaticbiochemicalanalyzer.⑦Glycatedhemoglobin(HbAlC)weretestedbyhighperformanceliquidchromatography(HPLC).⑧Insulinresistanceindex(HOMAd-IR)wascalculatedbyhomeostasismodelassessment(HOMAd-IR)=(FPG×FINS)/22.5.Results:①SerumsCD40LCRP,IL-6,TNF-α,VCAM-1levelsinIGRgroup,T2DMgroup,andT2DM+VCgroupweresignificantlyhigherthaninNCgroup.②sCD40L,IL-6,TNF-αmRNAexpressioninT2DMincreasedthaninIGRgroup,whiletheexpressionincreasedmoreinT2DM+VCgroup.③SerumVCAM-1levelsandIMT,IVST,LVPWTincreasedastheincreaseofsCD40LCRP,IL-6,TNF-αlevels,whileFMD,NIDdecreased.Especially,IVST,LVPWTlevelsinT2DMandT2DM+VCgroupweresignificantlyhigherthanintheotherones(P0.05).VCAM-1,IMT,FMD,NIDlevelsweresignificantlydifferentamongthesegroups(P0.05)④ThelevelsofsCD40Lwaspositivelyco