丙烯酰胺对大鼠神经行为的影响及褪黑素干预作用的分析-effect of acrylamide on neurobehavior in rats and analysis of melatonin intervention effect.docxVIP

与ACR组相比，MT+ACR组的尾长降低了39.1%（P 0.01），尾部DNA百分含量下降52.1%（P 0.01），Olive尾距降低56.8%（P 0.01）。结论： ACR 亚急性染毒能引起大鼠小脑细胞的 DNA 损伤，未观察到大脑皮层 细胞的 DNA 损伤， MT 同时干预可减轻 ACR 对小脑细胞的 DNA 损伤，但仍 未恢复到对照水平。综上所述，ACR亚急性经口中毒模型造成了大鼠神经功能的损伤及小脑的结 构病变，ACR对神经系统的损伤以功能损伤为主，结构损伤为辅。对生物大分子 的损伤表现为小脑的脂质过氧化损伤及DNA损伤，大脑皮层的脂质过氧化损伤 不明显，未观察到大脑皮层的DNA损伤，MT同时干预可以缓解ACR的神经毒作 用。氧化损伤可能介导ACR的神经毒作用，小脑的脂质过氧化损伤可能与结构损 伤有关，也可能与自由基或活性氧增多有关。小脑的DNA损伤可能是因为ACR 诱导产生大量的自由基或活性氧，直接攻击DNA导致氧化损伤。也可能是因为 小脑的脂质过氧化损伤引起神经细胞膜的通透性改变，使膜性结构受到破坏，诱 发DNA损伤，具体作用机制有待于进一步研究。关键词：褪黑素；丙烯酰胺；神经行为损伤；氧化损伤；DNA损伤Study of the intervention effect of Melatonin on ACR-Induced neurobehavioral damage in ratsCandidate for M.D.:Lanlan ZhuTutor：Hong YanAbstractAcrylamide (acrylamide) is a white crystalline substance and is precursor substance widely used in the production of polyacrylamide. It is mainly used for water purification treatment, pulp processing and so on. Starchy foods are prone to produceacrylamide at high temperatures cooking (120℃). Studies have shown that humanexposure to acrylamide through the digestive tract, respiratory tract, skin and mucous membrane and other ways.Acrylamide is detected in some fried and grilled starchy foods such as French fries, etc. The content is 500 times higher than the World Health Organization (WHO) allowed in drinking water (0.5μg / L).A large number of animal studies shows that acrylamide mainly cause neurotoxicity, reproductive and developmental toxicity and only neurotoxicity are proved to human.Neurotoxicity mainly are degenerative changes in peripheral nerve and central nervous system.The acute or subacute poisoning mainly shows central nervous system disorder, the chronic poisoning mainly shows peripheral neuropathy disorder,but the mechanism is still not fully elucidated.Melatonin (melatonin, MT) is the main hormone secreted by the pineal gland, it can regulate mood, sleeping, immunity, sex, reproduction and other physiological functions,and plays an important role in aging, tumor growth and the occurrence and developm