比格犬子宫与卵巢erα erβ的表达及erβ新剪接异构体的分析-expression of er α er β in beagle dogs uterus and ovary and analysis of er β new splicing isomers.docxVIP
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比格犬子宫与卵巢erα erβ的表达及erβ新剪接异构体的分析-expression of er α er β in beagle dogs uterus and ovary and analysis of er β new splicing isomers
缩略词表缩略词英文名称中文名称AFAmp APbp BLAST BSAERs ERE DEPC cDNAX-gal EB DMEM DMSOE.coli ERα ERβ LB RACEGAPDH IPTGKan LH FSH E2 PRGE GnRHGtH HRP PAGEPBSActivation Function Ampicillin Ammonium persulfate Base pairBasic Local Alignment Search Tool Bovine serumEstrogen receptors Estrogen Response Elements Diethyl pyrocarbonate Complementary deoxyribonucleic acid5-bromo-4-chloro-3-indoly-β-D-galactoside Ethidium BromideDulbecco’s modified Eagle’s medium Dimethyl sulfoxide Escherichia ColiEstrogen receptor α Estrogen receptor β Luria-BertaniRapid amplification of cDNA ends Glyceraldehyde-3-phosphate dehydrogenase Isopropylthio-β-D-galactosideKanamycin Luteinzing Hormone Follicle Stimulating hormone Estradiol Progesterone Gonadotropin releasing hormone Gonadotropic hormone Horseradish peroxidasePolyacrylamide gel electrophoresisPhosphate buffered saline活性功能区氨苄青霉素 过硫酸铵 碱基对基本的局部比对搜索工具 牛血清白蛋白雌激素受体 雌激素反应元件 焦碳酸二乙酯 互补脱氧核糖核酸5-溴-4-氯-3-吲哚半乳糖苷 溴化乙锭改良的 Eagle’s 培养基 二甲基亚砜大肠杆菌 雌激素受体 α 雌激素受体 β LB 培养基cDNA 末端快速扩增 甘油三磷酸脱氢酶 异丙基硫代-β-D 半乳糖苷 卡那霉素促黄体生成素 促卵泡素 雌二醇孕酮 促性腺激素释放激素 促性腺素 辣根过氧化物酶 聚丙烯酰胺凝胶电泳 磷酸盐缓冲液PCRRT-PCR SDS DBD LBD UV WB WTrpm IODTBSTPolymerase chain reactionReverse transcription-polymerase chain reaction Sodium dodecyl sulphateDNA binding domain Ligand binding domain Ultraviolet radiation Western blot Wide type Round per minute Integrated optical densityTris-buffered saline containing 0.05% Tween聚合酶链式反应反转录-聚合酶链式反应 十二烷基磺酸钠DNA 结合区 配体结合区 紫外照射 蛋白质印迹 野生型 每分钟转速 累积光密度Tris 缓冲盐溶液吐温TrismycTris(hydroxymethyl)metyl aminomethanemyc-tag三羟甲基氨基甲烷myc 癌基因标签蛋白肽DTT TEMED DAB AEC BCIP NBT GFPkD PMSFvsNP-40DithiothreitolN,N,N’,N’-Tetramethyl ethylenediamine 3,3’-diaminobenzidine3-amino-9-ethyl-carbozole5-bromo-4-chloro-3-indolyl-phosphate Nitro-blue-tetrazolium Green fluorescence protein Kilo-Dalton Phenylmethylsulfonyl fluorideversusNonidet P-40二硫苏糖醇N,N,N’,N’-四甲基乙二胺 二氨基联苯胺3-氨基-9-乙基咔唑5-溴-4-氯-3-吲哚-磷酸 四唑氮蓝 绿色荧光蛋白 千道尔顿 苯甲基黄酰氟…对…,与…比较 保湿剂中文摘要比格犬子宫与卵巢 ERα、ERβ 的表达及 ERβ新剪接异构体的研究比格犬因其性情温顺,体格适中,遗传背景清晰,均质性好,在医药、公共
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