Principles of measurement - Biomedical engineering 测量原理-生物医学工程.ppt

First Dilution 1:500 (WBC) Second Dilution 1:50.000 (RBC) WBC Dilution + 3 Drops Quicklyser for Lysing and HGB Measurement of particlesin a defined volume Calculation of cell-concentration with known Sample volume and defined Sample dilution no calibration of counting values Haemoglobin is the dye at the RBC. It is a Fe-containing Protein. Haemoglobin is exclusively produced by Erythroblasts in the bone marrow. Hemoglobin is an important part of the Erythrocytes. There is a direct relation between Haematokrit, Erythrocyte-concentration and Haemoglobin. The main function of Haemoglobin in the body is to transport Oxygen and CO2. A special reagent is needed, containing Cyanidefor lysing RBC F-Series = Quicklyser SLS means Sodiumlaurylsulfate SLS ins not toxic (ingredient in a lot of soaps tooth-paste) Sysmex Sulfolyser is now used with the majority of instruments (K-, E-, NE-,SF-,SE-, XE-systems) Only Sysmex hematology systems have a stable KCN-free Haemoglobin-measurement as standard. Hydrophobic area of SLS bind to Globin Conformation change Oxidation: Fe2+ -- Fe3+ Hydrophilic area of SLS bind to Fe3+ -- SLS-Hb. photometric measurement at 540 nm (F-Series, K-800, K-1000, K-4500) Haematocrit is determined by comparing the total or cumulative volume of red blood cells to the volume of whole blood. Determined normally by centrifugation or by electric pulse measurement Haematocrit is besides Haemoglobin an important parameter in anemia-diagnosis. Determining the volume of corpuscles in the blood Normally equivalent to mass of erythrocytes. Blood is drawn into special capillaries, which contain dried heparin. After closing, capillaries are placed into a centrifuge. In the centrifuge blood is divided into its corpuscular (cells) and fluid (plasma) components The auxiliary line through the borderbetween erythrocytes- and leukocytesgives the heamatocrit. CV app. 1% Erythrocyte-Indices Biological Variation Reliable Measurement Principles for