cd56galectin-3hbme-1ck19在甲状腺乳头状癌中表达及意义.docVIP

CD56、Galectin-3、HBME-1、CK19在甲状腺乳头状癌中的表达及意义 周浩锋 ，张会娟 郑州大学第一附属医院 河南郑州 471000摘要 目的 探讨神经黏附分子（CD56）、半乳糖凝集素3（Galectin-3）、人骨髓内皮细胞标记物(human bone marrow endothelial cell marker，HBME-1)和细胞角蛋白19(cytokeratin 19，CK19) 在甲状腺良、恶性病变鉴别诊断中的意义。方法 利用免疫组化S-P法检测96例甲状腺乳头状癌、28例结节性甲状腺肿和7例甲状腺腺瘤中CD56、Galectin-3、HBME-1和CK19蛋白表达。结果 在甲状腺乳头状癌中，CD56表达阴性率是87.5%，Galectin-3、HBME-1和CK19表达阳性率分别是93.8%、71%和95.8%；在甲状腺良性病变中，CD56表达阴性率是17.1%，Galectin-3、HBME-1和CK19表达阳性率分别是20%、11.4%和68.6%，四种抗体在甲状腺乳头状癌与甲状腺良性病变中的表达均具有显著性差异 (p0.05) 。结论CD56、Galectin-3、HBME-1、CK19是诊断甲状腺乳头状癌重要的分子标记物，联合检测可明显提高甲状腺乳头状癌的诊断准确度。 [关键词]：甲状腺乳头状癌；CD56；Galectin-3；HBME-1；CK19；免疫组化Abstract Objective To investigate the significance of neural adhesion molecule (CD56), galectin-3 (Galectin-3), human bone marrow endothelial cell markers (HBME-1) and cytokeratin 19 (CK19) in differentiating between thyroid benign and malignant lesions .Methods The expression of CD56，Galectin-3， HBME-1 and CK19 were detected in tissue sections from 96 cases of papillary thyroid carcinoma，28 cases of nodular goiter and 7 cases of thyroid adenoma by S-P immunohistochemical method. Results In papillary thyroid carcinomas, the negative rate of CD56 was 87.5% and the positive rates of Galectin-3, HBME-1 and CK19 were respectively 93.8%, 71% and 95.8%; In benign thyroid lesions, the negative rate of CD56 was 17.1 % and the positive rates of Galectin-3, HBME-1 and CK19 were respectively 20%, 11.4% and 68.6% .There were significant differences for CD56, Galectin-3, HBME-1 and CK19 expression between papillary thyroid carcinomas and benign thyroid lesions(P 0. 05 ). Conclusions CD56, Galectin-3, HBME-1and CK19 are important molecular markers for papillary thyroid cancer diagnosis，which combined can obviously improve the accuracy of the diagnosis of thyroid papillary carcinoma .Key words: papillary thyroid carcinoma ;CD56;Galectin-3;HBME-1;CK19;Immunohistochemistry甲状腺癌是内分泌系统最常见的恶性肿瘤之一，其中以甲状腺乳头状癌（papillary thyroid carcinomas，PTC）发病率最高。PTC病理诊断的典型特征：乳头状结构、沙粒体及特征性细胞核改变，如毛玻璃状核