张玲香第2次专业英语浅析.pptVIP

Cytogenetic study of a trigeneric hybrid (triticale × trtileymus) Name: Zhanglingxiang Major: Biological technology Grade:Grade2010 Class:Class1 Study purpose Plant material Research methods Research results Discussion Further research Purpose The study of alien chromosomes behavior in wheat background would benefit to assess the relationship between different genomes and understanding species phylogenies then help transfer alien genes into wheat. And the study benefits for the germplasm screening . Plant materials Octoploid triticale line(× Triticosecale c.v. Jinsong49,AABBDDRR, 2n = 8× = 56) Octoploid tritileymus(AABBDD(JN)(JN), 2n = 8× = 56) common wheat ‘Chinese Spring’, S. cereale, and L. mollis The method of the study 1.Observation of mitosis ※ Analysis the chromosome number of root tip cells . ※Observe if cells had the expected number of 28 bivalents. 2.Observation of mitosis ※Study meiosis of F1 hybrid, observe the meiotic configuration at MI . ※Study the chromosomes behavior of PMCs. 3.In situ hybridization ※Observe the chromosomes composition in the progenies of trigeneric hybrid. Results 1.Cytogenetics of the parental lines and the trigeneric hybrid ※ Both Jinsong49 and 950059 had the chromosome number with 2n = 56. Most examined cells had the expected number of 28 bivalents, few cells had univalents or multivalents. ※ The meiotic configuration at MI was 13.17I+20.82 II+0.37 III+0.02 IV. (Figure 1) A PMC of Trigeneric hybrid at diakinesis, most chromosomes at diakinesis located in the center of the cell, some chromatin remained in leptonema or interphase. (Figure 2) Among the 56chromosomes in Jinsong49 root tip cells at mitotic metaphase, there were 12 chromosomes entirely presenting greenish-yellow hybridization signals while the remaining chromosomes showed a uniform red fluorescence. probe: labeled genomic DNA of S.cereale blocking: genomic DNA of ‘Chinese Spring’ (Figure 3