石蜡切片原位杂交免疫组化3.doc

PAGE PAGE 82病 理 检 验 技 术江汉大学医学与生命科学学院病理学与病理生理学教研室 2004年4月内 容 提 要 本书介绍组织病理学常用的多种技术。包括常见的技术，即福尔马林固定、石蜡包埋、HE染色技术，同时也介绍了常用的特殊染色技术，免疫组织化学技术，并简明地介绍了分子生物学技术的基本理论以及在组织病理学中的应用。 全书理论与实践并重，经典与改良结合，内容丰富，通俗易懂，实用性强，为实验室工作的必备参考书。适合从事病理学、组织学、生物学、法医学等的科研教学和临床工作的技术人员、教师、医师和研究生使用。同时也适合临床医学检验专业中专、专科生使用以及临床医学专业专升本学生和临床医学专业本科生选用。石蜡切片技术组织标本的处理第一节 取材··············································5第二节 组织的固定········································6第三节 大体标本的处理和固定·····························14第四节 陈列标本的固定···································15第五节 脱钙·············································16第六节 组织的冲洗·······································18石蜡包埋技术第一节 脱水··············································19第二节 透明··············································21第三节 浸蜡··············································22第四节 包埋··············································22第三章 切片技术第一节 切片刀············································25第二节 切片机············································27第三节 石蜡切片的制作····································27第四节 特殊组织石蜡切片的制作····························29第五节 石蜡切片的异常及处理······························29第四章 染色与染色剂第一节 染色的基本原理····································31第二节 染色剂染色的化学基础······························33第三节 染色剂的分类······································34第四节 常用染色剂及配制··································35第五节 苏木素—伊红染色法································39第六节 封固剂············································41第五章 特殊染色技术第一节 结缔组织染色法····································43第二节 脂类染色法·······································47第三节 糖原及粘液染色法··································48第四节 色素染色法········································50第五节 神经组织染色法····································52第六节 核酸及核蛋白染色法································52第七节 肌肉组织染色法····································53第八节 病原微生物染色法··································54第九节 特殊染色技术的应用································57第二篇 免疫组织化学和亲和免疫组织化学技术第一章 免疫组织化学技术第一节 基本原理··········································62第二节 染色步骤···································