脐血干细胞移植对兔后肢动脉内膜损伤后修复及血清vegf tgf-β1水平的影响-effects of umbilical cord blood stem cell transplantation on repair of rabbit hind limb artery intima injury and serum vegf tgf - β 1 level.docxVIP

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脐血干细胞移植对兔后肢动脉内膜损伤后修复及血清vegf tgf-β1水平的影响-effects of umbilical cord blood stem cell transplantation on repair of rabbit hind limb artery intima injury and serum vegf tgf - β 1 level.docx

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脐血干细胞移植对兔后肢动脉内膜损伤后修复及血清vegf tgf-β1水平的影响-effects of umbilical cord blood stem cell transplantation on repair of rabbit hind limb artery intima injury and serum vegf tgf - β 1 level

目录中文摘要············································································································1英文摘要············································································································3英文缩写············································································································6研究论文脐血干细胞移植对兔后肢动脉内膜损伤后修复及血清VEGF、TGF-β1水平的影响前言7材料与方法································································································8结果··········································································································11附图··········································································································12附表··········································································································15讨论··········································································································16结论··········································································································19参考文献··································································································20综述脐血干细胞移植及血管成形治疗糖尿病下肢缺血性疾病··············23致谢··················································································································34个人简历··········································································································35脐血干细胞移植对兔后肢动脉内膜损伤后修复及血清VEGF、TGF-β1水平的影响摘要目的：观察兔后肢动脉经球囊扩张造成急性动脉内膜缺血损伤模型后，人脐血干细胞局部移植（humancordbloodstemcelltransplantation，HCBSCT）对兔血清中血管内皮生长因子（vascularendothelialgrowthfactor，VEGF）及转化生长因子-β1（transforminggrowthfactorbeta-1，TGF-β1）水平的影响和损伤血管组织病理学变化，探讨脐血干细胞移植对防治血管成形术后再狭窄的可能机制。方法：12014年12月至2015年2月，新西兰大白兔18只随机分入对照组（A组，n=6）、单纯球囊损伤组（B组，n=6），及球囊损伤+人脐血干细胞移植组（C组，n=6）。采用Ficoll密度梯度离心法分离制备人脐血单个核细胞悬液，分离后在2小时内用完。2A组仅分离暴露左侧后肢动脉，穿刺局部注入0.5ml磷酸盐缓冲液（PBS缓冲液），标记后闭合伤口；C组行动脉分离后，夹闭暴露的动脉两端，穿刺成功以3.0mm×40mm球囊进行局部扩张并间断牵拉，每次30s，共3次，成功制备急性动脉内膜缺血损伤模型后，经球囊导管注入脐血干细胞悬液0.5ml(2×106个)于后肢动脉损伤局部，缓慢撤出球囊，缝合穿刺位点，约20min后撤去