结核分枝杆菌的形态染色.PPTVIP

掌握：结核分枝杆菌的形态染色、培养特性、抵抗力、致病机理、结核菌素试验的原理与用途、微生物学检查法、卡介苗预防 熟悉：结核分枝杆菌的免疫与变态反应关系。 chapter 20 mycobacteria Acid Fastness Stain(Ziehl-Neelsen stain) flood the slide with basic fuchsin (a red dye) in 5% phenol as a mordant. heat gently for few minutes to melt the wax. wash with 3% HCl in ethanol. counter-stain with methylene blue. Mycobacterium stains red and other bacteria and the background are blue. The mycolic acid are responsible for the acid fastness. Mycobacterium tuberculosis Common features aerobic, acid-fast rods inability to be Gram-stained resist decolorization wi