临床医学实时荧光定量PCR在临床医学中的应用ppt课件.pptVIP

Novel Signal-Improved HRM DyeProvided in High-Resolution Melting Master This picture shows High-Resolution Melting Curve data generated with the HRM dye in comparison to SYBR Green I. The HRM dye binds to double-stranded DNA in a saturating fashion. It gives higher signals and very sharp and distinct melting signals that, can resolve very subtle differences between e.g., wildtype and heterozygous samples in high resolution melting. SYBR Green I is not suited for this purpose. The Roche HRM dye is provided in the LightCycler? 480 High Resolution Melting Master. It‘s novel and proprietary