Preventionofviralinfection病毒感染的免疫预防.PPTVIP

Diagnosis, prevention and treatment Xiao-Kui Guo PhD Content Lab diagnosis of the viral infection Prevention of the viral infection Anti-virus treatment Section ALab Diagnosis of the viral infection Methods of the diagnosis Diagnostic virology (病毒感染的检查方法)provides evidence for the physician to make right diagnosis, as well as the treatment scheme. virus isolation identification and confirmation(病毒分离与鉴定) viral nucleic acid amplification techniques(病毒核酸检测技术) viral antigen or specific antibodies detection（病毒抗原及特异性抗体的检测） Specimen Collection and Shipment （标本的采集与送检） A. Samples for Virus Isolation and Viral Nucleic Acid Amplification 1. Timeliness of Specimen Collection 2. Sample Type 3. Sample shipment 4.Process and Stock Samples B. Samples for Serology Detection Virus Isolation A. Cell Culture B. Animal Inoculation- inoculating a susceptible host (laboratory) animal with infectious material mice, guinea pigs（豚鼠）, rabbits, monkeys, and ferrets（雪貂） C. Chick Embryo Inoculation Chorioallantoic membrane (绒毛尿囊膜) (CAM) ①titration of viruses ②investigation of viral morphology ③identification of viruses Allantoic sac(尿囊腔) -required large quantities of viruses Amniotic sacs (卵黄囊)- the isolation of various viral agents A. Cell Culture 1. Cell culture category (1) Primary cell culture (2) Diploid cell culture (3) Continuous Cell Culture (4) Lymphocyte culture 2. Virus Identification (1) Characterization of Viral Intracellular Propagation 1) Cytopathogenic Effect（细胞致病作用）(CPE) 2) Hemadsorption phenomenon 3) Viral Interference (2) Quantification of Viral Infection 1) Plaque-forming Unit（空斑形成单位）(PFU) 2) The Tissue Culture Infectious Dose50 (50%致死量（LD50）或50%组织细胞感染量) (TCID50) Assay 3) Virus Morphology and Structure (病毒形态与结构)Observation 3. Viral Antigen Detection Directly Detect Viral Antigen Measure viruses without permissive cell line (病毒敏感细胞系) A. Immunofluorescent Assay Immunofluorescent microscopy（荧光显微镜） B. Enzyme-Linked Immunosorbent Assays (ELISA) Direct Det