十全大补汤配方颗粒对骨髓抑制小鼠造血调控的实验分析-experimental analysis of shiquan dabu decoction formula granule on hematopoietic regulation of bone marrow suppression mice.docxVIP

成都中医药大学博士学位论文 成都中医药大学博士学位论文 Shiquandabu Tang Granules could promote bone marrow cells into the cell cycle, promote the G0/G1 phase cells into S phase, S phase cells into G2/M phase transformation, thereby promoting the proliferation of bone marrow cells. Shiquandabu Tang Granules could promote gene expression of cell cycle regulatory protein (CyclinD1, CyclinE) and cyclin-dependent kinase (CDK2, CDK4); Shiquandabu Tang Granules could promote gene expression of EPOmRAN in Kidney and BMC, TPOmRNA in spleen, GM-CSFmRNA in BMC, increase the level of serum EPO, TPO and GM-CSF;. Shiquandabu Tang Granules could promote early hematopoietic stem / progenitor cells adhesion and homing by increasing the adhesion of bone marrow stromal cells, promoting the expression of FN, LN, and inhibiting the expression of MMP-2, MMP-7, MMP-9 in extracellular matrix of bone marrow. Conclusion: Within a certain dose range, Shiquandabu Tang Granules could promote effectively the recovery of impaired hematopoietic function in myelosuppressed mice . The main channels may be regulating cell cycle of BMC, improving the bone marrow micro-environment, promoting the gene expression of hematopoietic growth factors. There has no significant difference between the Shiquandabu Tang Granules and the traditional decoction in improving the bone marrow hematopoietic function of myelosuppressed mice. Key words: Shiquandabu Tang Granules Myelosuppressed mice Cell cycle Hematopoietic microenvironment Hematopoietic growth factors 4 缩略词表 英文缩写 英 文 中 文 AP Ammonium persulfate 过硫酸铵 Acr acrylamide 丙烯酰胺 BMC Bone marrow cell 骨髓细胞 BMNC Bone marrow nucleated cell 骨髓有核细胞 BMSCs Bone marrow stromal cells 骨髓基质细胞 BFU-E Burst forming unit-erythroid 早期红系祖细胞 CH chloromycetin 氯霉素 CFU-E Colony forming unit-erythroid 晚期红系祖细胞 Colony forming unit-granulocyte- CFU-GM  macrophage 粒-单核系祖细胞 CFU-M Colony forming unit-macrophage 单核巨噬系集落形成单位 CFU-Meg Colony forming unit- megakaryocyte 巨核系祖细胞 CSA Colony sti