人禽流感疫苗研发进展ppt课件.ppt

Vaccines for Pandemic Influenza流感大流行疫苗储备株研究进展; Family: Orthomyxoviridae（正粘病毒科） Three types: A, B, C SS negative-sense RNA viruses （单股副链RNA病毒） 8 (or 7) gene segments（8或7节段） Enveloped viruses （囊膜病毒）;Influenza A Viruses;Antigenic Drift（抗原漂变）;Antigenic Shift（抗原转换）;17 HA subtypes;What can cause Pandemic Influenza? ;Human infection with avian influenza viruses(~2004)人感染禽流感病毒情况;From （禽流感H5N1全球感染情况）;H5N1 influenza in animals other than poultry -2004除家禽以外感染H5N1的动物 ; Cumulative Number of Confirmed Human Cases of Avian Influenza A/(H5N1) Reported to WHO 14 August 2007 （WHO确诊人禽流感病例） ;WHO 流感大流行预警3级; Number of Episodes of Illness, Healthcare Utilization, and Death Associated with Moderate and Severe Pandemic Influenza Scenarios;Options for Vaccines for Pandemic Influenza 流感大流行疫苗株的选择;Choice of Vaccines: Pros and Cons疫苗选择的优缺点;PR8(H1N1);Bi-directional plasmids that express both mRNA (for protein expression) and vRNA;Vaccine against H5N1 viruses（H5N1病毒疫苗）1. HK97 strains;2. 2003-2004 H5N1 virus;3. 2005-2006 H5N1 viruses; VietnamHN3040805;Vaccines against other subtypes （其它亚型流感病毒疫苗）;Procedures of pandemic vaccine（疫苗研发流程）;Growth Characteristics of reassortant virus; plaque forming capability in CEF cells with or without trypsin（重配株的生长特性，空斑形成能力） Genotype and sequence of the HA and NA genes（HA，NA序列分析和基因分型） Pathogenicity in chickens （对鸡致病性） Pathogenicity in mammals (mice and/or ferrets)（哺乳动物致病性包括鼠和/或雪貂） Antigenicity of the reassortant vaccine reference virus （HI）（重配疫苗病毒抗原性检测） Efficacy of protection from challenge with wild-type virus in mouse model（野毒攻击检测疫苗的保护性） ;d60;Protection of H5 immunised mice against WT virus challenge（疫苗的免疫保护性评价）;Acknowledges