决明子中黄酮类化合物提取方法论文.doc

PAGE III 摘 要 决明子是一种常用中药，富含黄酮，蒽醌和多糖，具有广泛的药理活性。现代药理学研究又表明，决明子具有降血压、降血脂，保肝及抑菌等活性，被应用于多种降脂减肥的成方制剂中。同时，决明子又可作食用中药，是保健饮料的良好原料。 实验过程中测定了决明子中黄酮类化合物的含量，采用乙醇回流水浴提取黄酮，对比研究提取时间、乙醇浓度、浸出温度和料液比等因素对提取效果的影响,再进行正交实验优化工艺条件。含量测定应用紫外—可见分光光度法进行测定，并使用NaNO2-Al(NO3)3-NaOH显色体系。 实验结果表明乙醇提取法效果最佳。在最佳提取条件75％乙醇为提取剂，80℃下提取，提取时间3.5h，料液比1:30时。以芦丁为标准物质，绘制的标准标准曲线其回归方程为C=96.838A-2.8675，相关系数R2=0.9951。又测得提取液吸光度为0.428，黄酮浓度为37.0μm/mL，提取率为88.61% 关键词：决明子，黄酮类化合物，乙醇，提取工艺 Abstract Semen Cassiaen a traditional Chinese herbal medicine, which is a rich source of flavones,polysaccharides and anthraquinone,having a widespread activity in biology. Modern pharmacology study also shows that, cassia seed also have fall blood pressure, fall hematic fat, protect liver and bacteriostasis activities, be applied to various cholesterol-lowering diet drugs in preparations. Meanwhile, cassia seed and eating Chinese traditional medicine, is the good health drink raw material.Some chemical separation factors were adopted to extract flavonoids. This experiment determined the content of flavonoids in cassia seed, and cassia seed the extraction technology of flavonoids were studied.Ethanol Condensing Reflux Extraction were applied to extract flavonoids．When extracting，Five extraction factors of flavonoids from Semen Cassiaen were investigated and compared, including extracting time ，ethanol concentration，extracting temperature and ratio of liquid to solid. Again the optimization of process conditions for orthogonal experiment. Quantification was performed by means of UV–Vis spectrophotometry with chromogenic system of NaNO2–Al (NO3)3–NaOH. Experiments show that that the ethanol extraction was better than the other．The found optimum extraction conditions were that the ethanol concentration was 75% (v/v), solid–liquid ratio was 1:30, extracting temperature was 80°C, extracting time was3.5h. Using the concentration of rutin standard solution as abscissa and the absorbency as y-coordinate, the linear chart was constructed and the sta