胃癌chfr、runx3基因启动子区异常甲基化的研究-aberrant methylation of chfr and runx 3 gene promoter regions in gastric cancer.docxVIP

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胃癌chfr、runx3基因启动子区异常甲基化的研究-aberrant methylation of chfr and runx 3 gene promoter regions in gastric cancer.docx

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胃癌chfr、runx3基因启动子区异常甲基化的研究-aberrant methylation of chfr and runx 3 gene promoter regions in gastric cancer

安徽医科大学硕士学位论文安徽医科大学硕士学位论文 PAGE PAGE 6 Study on Aberrant Promoter Methylation of CHFR and RUNX3 Genes in Gastric Cancer Abstract Objective In this study, the methylation level of CHFR and RUNX3 genes promoter in gastric carcinoma tissues, 5cm adjacent nonmalignant gastric mucosa tissues and normal gastric mucosa, pre-operative and post-operative serum, were detected by combination of methylation-specific polymerase chain reaction (MSP) and combined bisulfite restriction analysis (COBRA), and to explore the relationship between the aberrant methylation of these two genes and the clinical parameters of the gastric cancer. Then, we compare the difference of MSP and COBRA on the methylation detecting of tumor suppressor gene in gastic cancer. Methods Patients diagnosed as primary gastric cancer without receiving any radiotherapy or chemotherapy before operation were collected. A total of 123 carcinoma specimens, the matched neighboring tissues during their surgical resection, and thirty normal gastric mucosa were collected as control group.78 pre-operative and corresponding post-operative sera were taken from the patients. The techniques of MSP and COBRA was adopted to investigate the promoter methylation of CHFR gene in 64 carcinoma specimens . Results The aberrant methylation of CpG islands of CHFR gene promoter The positive rate of methylation of CHFR gene was found to be 41.5% (51/123) in gastric cancer tissues,11.4% (14/123) in the matched neighboring tissues and 0% (0/30) in normal gastric mucosa.The positive rate of methylation of CHFR gene in the former group was significantly higher than that in the latter two groups (P＜0.05). The positive rate of methylation of CHFR gene from degree of histological differentiation G3/G4 carcinoma tissues was significantly higher than that from the tissues samller than G1/G2 (P＜0.05). And the positive rate of methylation of CHFR gene from diameter lager than 5cm was significantly higher than that from the tissues samller th