食管鳞癌细胞中rictor的表达情况及抑制rictor对akt和细胞增殖影响-expression of ri vector in esophageal squamous carcinoma cells and its effect on akt and cell proliferation.docxVIP

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食管鳞癌细胞中rictor的表达情况及抑制rictor对akt和细胞增殖影响-expression of ri vector in esophageal squamous carcinoma cells and its effect on akt and cell proliferation.docx

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食管鳞癌细胞中rictor的表达情况及抑制rictor对akt和细胞增殖影响-expression of ri vector in esophageal squamous carcinoma cells and its effect on akt and cell proliferation

万方数据万方数据摘要 Rictor mRNA的表达明显降低。转染Rictor-shRNA后，pp242对ECa109细胞增殖的抑制作用更加明显。结论 1.食管鳞癌中Rictor的表达与细胞分化无相关性；食管鳞癌细胞株中雷帕霉素对 mTORC1作用明显，pp242对mTORC1和mTORC2都有较好的抑制作用。 2.当RNA干扰Rictor表达后，ECa109细胞对pp242更敏感。关键词：食管鳞癌、RNA干扰、Rictor、雷帕霉素、pp242 II Abstract Abstract Purpose This study will help explore rapamycin and pp242 on the role of mTORC1 and mTORC2 relations,to uncover rapamycin and pp242 anti-cancer mechanism foundation, and deep understanding of Rictor as mT0RC2 members in its role in the development of tumor, in order to guide the biological target therapy for malignant tumor. Method MTT method to detect pp242 proliferation inhibition of esophageal squamous cancer cells. Western-blot were used to detect different degrees of differentiation of five strains of esophageal squamous carcinoma cell line:TE-1, EC9706, KYSR450, KYSR750, Eca109. The protein expression of Rictor, p-Akt (Ser473), Raptor, p-p70S6K were detected in mTOR pathway, Rictor expression and cancer differentiation correlation was researched, then with different concentrations and time point of rapamycin and pp242 effects on different esophageal squamous cancer cells, to observe both of mTOR pathway Rictor, p-Akt (Ser473), Raptor, p-p70S6K protein expression. 3.Rictor-shRNA plasmid transfection Eca109 cells, by puromycin filter to get stable expression cell lines. To detect the interference effect by RT-PCR and western-blot method,and then determined by MTT method to detect pp242 impact on ECa109 cell proliferation interference Rictor before and after. Result MTT results showed pp242 cells proliferation inhibition of esophageal squamous cancer cells. western-blot showed that, Rictor expression in the esophageal carcinoma cell lines do exist differences in expression, in poorly differentiated seedlings EC9706 and high differentiation seedlings ECa109 expressed in high volume than other cell lines. Raptor, p-p70S6K with varying degrees of lower in the cell were treated with III Rapamyci