REM―PCL对犬缺血再灌注心肌线粒体抗氧化能力影响.docVIP

REM―PCL对犬缺血再灌注心肌线粒体抗氧化能力影响 　　【摘要】 目的：观察REM-PCL对体外循环（CPB）中犬心肌线粒体抗氧化能力的影响。方法：采用CPB心肌缺血再灌注模型，12只犬随机分为REM-PCL组（RP组，n=6）和对照组（C组，n=6）。RP组和C组分别于CPB前静脉注射0.2 mg/kg REM-PCL及等量生理盐水。两组分别于转机前、缺血60 min、再灌注30 min和60 min时，检测总抗氧化能力（T-AOC）、谷胱甘肽过氧化物酶（GSH-PX）、丙二醛（MDA）、超氧化物歧化酶（SOD）和心肌线粒体线粒体肿胀度（MSD）。结果：与转机前比较，两组缺血后T-AOC、GSH-PX及SOD均降低，MDA和MSD含量均升高（P0.05）。缺血60 min和再灌注后RP组MDA和MSD含量均显著低于C组（P0.01），T-AOC、GSH-PX及SOD均显著高于C组（P0.01）。结论：REM-PCL预处理可通过提高CPB中心肌线粒体抗氧化能力来减轻心肌线粒体损伤。 　　【关键词】 瑞芬太尼聚己内酯； 线粒体； 体外循环 　　【Abstract】 Objective：To observe the effect of remifentanil-poly-caprolactone（REM-PCL）on the ability of anti-lipid peroxidation of canine myocardial mitochondrial during cardiopulmonary bypass（CPB）.Method：Twelve healthy dogs undergoing CPB were randomly allocated into control group（group C，n=6）and REM-PCL pretreatment group（group RP，n=6）.All the dogs were anesthesitized with 2.5% sodium pentobarbitone 25mg/kg.After tracheal intubation，the myocardial ischemia-reperfusion injury model during CPB was made by declamping（DC）after aortic cross-clamping（AC）for 60 min.In group P，REM-PCL 0.2 mg/kg was administered intravenously before CPB while in group C physiological saline was given instead of REM-PCL.Myocardial mitochondria were isolated before CPB，60min after AC，30 min and 60 min after DC for determination of mitochondrial swelling degree（MSD）， malondialdehyde（MDA），glutathione peroxidase（GSH-PX），superoxide dismutase（SOD）and total anti-oxidation capacity（T-AOC）.Result：The myocardial mitochondria of MSD and MDA were increased while GSH-PX，SOD and T-AOC were decreased after AC as compared to the value before CPB in both groups（P0.05）.MSD and MDA were distinctly higher while GSH-PX，SOD and T-AOC were distinctly lower at 60min after AC and after DC in group RP than those in group C（P0.01）.Conclusion：Pretreatment with REM-PCL is effective in improving the anti-oxidation ability of myocardial mitochondria and attenuate myocardial mitochondrial injury. 　　【Key words】 Remifentanil-poly-caprolactone； Mitoch