COPD患者支气管肺泡灌洗液中IL―22检测及意义.docVIP

COPD患者支气管肺泡灌洗液中IL―22检测及意义 　　【摘要】 目的：检测慢性阻塞性肺病（COPD）患者急性肺炎链球菌感染后支气管肺泡灌洗液（BALF）IL-22的含量，并探讨其临床意义。方法：采用酶联免疫吸附试验（ELISA）检测30例急性肺炎链球菌感染的COPD患者（COPD组）和15例急性肺炎链球菌感染性支气管炎（SP）患者（SP组）BALF上清中IL-22的含量，并对10例患者进行了治疗后跟踪检测，另选取15例健康志愿者作为健康对照组（HC组）。结果：COPD组、SP组患者BALF中IL-22的含量均明显高于健康对照组（P0.001）；且SP组患者BALF中IL-22的含量明显高于COPD组（P0.01）。治疗后，COPD组患者BALF中IL-22的含量明显下降，吸烟患者IL-22的含量明显高于不吸烟患者（P0.01）。COPD患者IL-22的含量与中性粒细胞在BALF中的比例呈正相关关系（P0.01）。COPD患者IL-22的含量与FEV1%呈负相关关系（P0.01）。结论：COPD患者BALF中IL-22的含量增加与COPD的发生与发展密切相关。 　　【关键词】 慢性阻塞性肺病； IL-22； 灌洗液 　　Detection and Significance of IL-22 in Bronchoalveolar Lavage Fluid of Patients with COPD/XU Xi-rong.//Medical Innovation of China，2017，14（27）：005-009 　　【Abstract】 Objective：To investigate the level of IL-22 in BALF of patients with COPD infected pneumonia streptococcus and explore its clinical significance.Method：Enzyme linked immunosorbent assay （ELISA） was used to detect the content of BALF in the supernatant of IL-22 in 30 patients with COPD（COPD group） and 15 patients with acute streptococcus pneumoniae （SP）（SP group） infection bronchitis，and 10 patients were followed up after treatment，15 healthy volunteers served as healthy control group （HC group）.Result：The content of IL-22 in BALF of COPD group and SP group were significantly higher than that in healthy control group（P0.001）；the content of IL-22 in BALF of SP group was significantly higher than that in COPD group（P0.01）.After treatment，the content of IL-22 in BALF of COPD group decreased obviously，and the content of IL-22 in smokers was higher than that in non smokers（P0.01）.There was a positive correlation between the content of IL-22 and the proportion of neutrophils in BALF of COPD patients（P0.01）.The content of IL-22 in patients with COPD was negatively correlated with FEV1%（P0.01）.Conclusion：In patients with COPD，the increase of IL-22 in BALF is closely related to the occurrence and development of COPD. 　　【Key words】 COPD； IL-22； BALF 　　First-author’s address：The Central Hospital of Sha